دورية أكاديمية
Methylation alterations of imprinted genes in different placental diseases.
| العنوان: | Methylation alterations of imprinted genes in different placental diseases. |
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| المؤلفون: | Wang X; Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, No.63 Duobao Road, Liwan District, Guangzhou, 510150, Guangdong, China.; Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Liu Y; Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Wu Y; The Third Clinical College of Guangzhou Medical University, Guangzhou, China., Lin C; The Third Clinical College of Guangzhou Medical University, Guangzhou, China., Yang S; Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, No.63 Duobao Road, Liwan District, Guangzhou, 510150, Guangdong, China.; Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.; BioResource Research Center, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Yang Y; Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, No.63 Duobao Road, Liwan District, Guangzhou, 510150, Guangdong, China.; Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Chen D; Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, No.63 Duobao Road, Liwan District, Guangzhou, 510150, Guangdong, China.; Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Yu B; Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, No.63 Duobao Road, Liwan District, Guangzhou, 510150, Guangdong, China. yubolan-q@qq.com.; Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. yubolan-q@qq.com.; BioResource Research Center, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. yubolan-q@qq.com. |
| المصدر: | Clinical epigenetics [Clin Epigenetics] 2024 Sep 18; Vol. 16 (1), pp. 132. Date of Electronic Publication: 2024 Sep 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Biomed Central Country of Publication: Germany NLM ID: 101516977 Publication Model: Electronic Cited Medium: Internet ISSN: 1868-7083 (Electronic) Linking ISSN: 18687075 NLM ISO Abbreviation: Clin Epigenetics Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oct./Nov. 2011- : London : Biomed Central Original Publication: Berlin : Springer-Verlag |
| مواضيع طبية MeSH: | DNA Methylation*/genetics , Genomic Imprinting*/genetics , Insulin-Like Growth Factor II*/genetics , Pre-Eclampsia*/genetics, Humans ; Female ; Pregnancy ; Adult ; GTP-Binding Protein alpha Subunits, Gs/genetics ; Placenta/metabolism ; Epigenesis, Genetic/genetics ; Hydrocortisone/blood ; Placenta Diseases/genetics ; Oxidative Stress/genetics ; Fetal Blood/chemistry ; Fetal Blood/metabolism ; Chromogranins ; Proteins ; Potassium Channels, Voltage-Gated |
| مستخلص: | Background: Imprinted genes play important functions in placentation and pregnancy; however, research on their roles in different placental diseases is limited. It is believed that epigenetic alterations, such as DNA methylation, of placental imprinting genes may contribute to the different pathological features of severe placental diseases, such as pre-eclampsia (PE) and placenta accreta spectrum disorders (PAS). Results: In this study, we conducted a comparative analysis of the methylation and expression of placental imprinted genes between PE and PAS using bisulfite sequencing polymerase chain reaction (PCR) and quantitative PCR, respectively. Additionally, we assessed oxidative damage of placental DNA by determining 8-hydroxy-2'-deoxyguanosine levels and fetal growth by determining insulin-like growth factor 2 (IGF2) and cortisol levels in the umbilical cord blood using enzyme-linked immunosorbent assay. Our results indicated that methylation and expression of potassium voltage-gated channel subfamily Q member 1, GNAS complex locus, mesoderm specific transcript, and IGF2 were significantly altered in both PE and PAS placentas. Additionally, our results revealed that the maternal imprinted genes were significantly over-expressed in PE and significantly under-expressed in PAS compared with a normal pregnancy. Moreover, DNA oxidative damage was elevated and positively correlated with IGF2 DNA methylation in both PE and PAS placentas, and cortisol and IGF2 levels were significantly decreased in PE and PAS. Conclusions: This study suggested that DNA methylation and expression of imprinted genes are aberrant in both PE and PAS placentas and that PE and PAS have different methylation profiles, which may be linked to their unique pathogenesis. (© 2024. The Author(s).) |
| References: | Nature. 1993 Nov 25;366(6453):362-5. (PMID: 8247133) Am J Obstet Gynecol. 2017 May;216(5):527.e1-527.e9. (PMID: 28043842) Nature. 2004 Nov 4;432(7013):53-7. (PMID: 15525980) Curr Drug Targets. 2015;16(1):13-9. (PMID: 25585126) PLoS One. 2012;7(11):e49248. (PMID: 23145134) Reprod Sci. 2013 Aug;20(8):968-80. (PMID: 23302396) J Physiol. 2017 Aug 1;595(15):5057-5093. (PMID: 28337745) Clin Genet. 2012 Apr;81(4):341-9. (PMID: 22236068) Hum Reprod Update. 2019 Nov 5;25(6):777-801. (PMID: 31633761) Reprod Sci. 2014 Dec;21(12):1508-17. (PMID: 24803508) Mol Cell Endocrinol. 2013 Nov 5;380(1-2):65-78. (PMID: 23523966) J Gastroenterol. 2022 Mar;57(3):208-220. (PMID: 35018527) Clin Epigenetics. 2020 May 24;12(1):71. (PMID: 32448196) Endocrinology. 2010 Feb;151(2):741-7. (PMID: 20032056) Oxid Med Cell Longev. 2021 Aug 14;2021:9912436. (PMID: 34426760) Mediators Inflamm. 2021 Sep 27;2021:9962860. (PMID: 34616234) BMC Genomics. 2013 Jul 12;14:472. (PMID: 23844573) Biochem Pharmacol. 2019 Nov;169:113628. (PMID: 31491415) Nature. 1985 Jun 6-12;315(6019):496-8. (PMID: 4000278) Environ Int. 2019 Mar;124:482-492. (PMID: 30684806) Cell Mol Life Sci. 2020 Jun;77(11):2091-2101. (PMID: 31813015) Environ Pollut. 2021 Aug 15;283:117137. (PMID: 33866218) Adv Exp Med Biol. 2015;872:127-44. (PMID: 26215993) Mod Pathol. 2020 Dec;33(12):2382-2396. (PMID: 32415266) Cancer Med. 2018 Aug;7(8):4012-4022. (PMID: 29989329) Genes (Basel). 2020 Sep 29;11(10):. (PMID: 33003346) Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):15852-15861. (PMID: 32576693) Chem Biol Interact. 2014 Feb 5;208:77-83. (PMID: 24296128) J Reprod Immunol. 2011 May;89(2):126-32. (PMID: 21529966) PLoS One. 2015 Aug 04;10(8):e0135202. (PMID: 26241757) Pediatr Dev Pathol. 2017 Sep-Oct;20(5):387-393. (PMID: 28812469) Epigenetics Chromatin. 2022 Jan 21;15(1):3. (PMID: 35063005) Epigenetics. 2015;10(1):50-61. (PMID: 25496377) Clin Epigenetics. 2019 Aug 1;11(1):113. (PMID: 31370882) Hypertension. 2011 Sep;58(3):497-504. (PMID: 21730298) Curr Opin Pediatr. 2020 Dec;32(6):719-729. (PMID: 33148967) |
| معلومات مُعتمدة: | 2022YFC2704500 National Key Research and Development Program of China; SL2022A03J00860 Guangzhou Science, Technology and Innovation Commission |
| فهرسة مساهمة: | Keywords: Imprinted genes; Methylation; Placenta accreta spectrum disorders; Pre-eclampsia |
| المشرفين على المادة: | 67763-97-7 (Insulin-Like Growth Factor II) 0 (IGF2 protein, human) EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gs) EC 3.6.1.- (GNAS protein, human) WI4X0X7BPJ (Hydrocortisone) 0 (mesoderm specific transcript protein) 0 (KCNQ1OT1 long non-coding RNA, human) 0 (Chromogranins) 0 (Proteins) 0 (Potassium Channels, Voltage-Gated) |
| تواريخ الأحداث: | Date Created: 20240918 Date Completed: 20240919 Latest Revision: 20240921 |
| رمز التحديث: | 20240921 |
| مُعرف محوري في PubMed: | PMC11409545 |
| DOI: | 10.1186/s13148-024-01738-3 |
| PMID: | 39294759 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1868-7083 |
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| DOI: | 10.1186/s13148-024-01738-3 |