دورية أكاديمية
GNAS, not a Highly Mutated Gene, Has Prognostic Significance and Carcinogenic Effects in Osteosarcoma.
| العنوان: | GNAS, not a Highly Mutated Gene, Has Prognostic Significance and Carcinogenic Effects in Osteosarcoma. |
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| المؤلفون: | Qi J; Department of Orthopedics, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu 241001, Anhui Province, China., Huang Y; Human Anatomy Experimental Training Center, School of Basic Medicine Science, Wannan Medical College, Wuhu 241001, Anhui Province, China., Bian Y; Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai 201400, China, :15370890937@163.com. |
| المصدر: | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology [Cell Physiol Biochem] 2024 Sep 15; Vol. 58 (5), pp. 510-526. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Physiol Biochem Press GmbH & Co KG Country of Publication: Germany NLM ID: 9113221 Publication Model: Print Cited Medium: Internet ISSN: 1421-9778 (Electronic) Linking ISSN: 10158987 NLM ISO Abbreviation: Cell Physiol Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Düsseldorf, Germany : Cell Physiol Biochem Press GmbH & Co KG Original Publication: Basel ; New York : S. Karger, 1991-2018. |
| مواضيع طبية MeSH: | Osteosarcoma*/genetics , Osteosarcoma*/pathology , Osteosarcoma*/metabolism , GTP-Binding Protein alpha Subunits, Gs*/genetics , GTP-Binding Protein alpha Subunits, Gs*/metabolism , Chromogranins*/genetics , Chromogranins*/metabolism , Mice, Nude* , Bone Neoplasms*/genetics , Bone Neoplasms*/pathology , Bone Neoplasms*/metabolism, Humans ; Animals ; Cell Line, Tumor ; Prognosis ; Mice ; Male ; Female ; Mutation ; RNA, Small Interfering/metabolism ; Cell Proliferation ; RNA Interference ; Apoptosis ; Mice, Inbred BALB C ; Carcinogenesis/genetics ; Carcinogenesis/pathology |
| مستخلص: | Background/aims: Osteosarcoma is a prevalent and aggressive primary malignant bone tumor affecting children and adolescents. Despite advancements in sequencing technologies, there remains a lack of reliable prognostic biomarkers and effective targeted therapies for osteosarcoma. This study focuses on identifying key prognostic genes, particularly the role of GNAS, in osteosarcoma progression. Methods: Bioinformatics analyses were performed on osteosarcoma datasets from the Gene Expression Omnibus (GEO). Differential gene expression analysis, weighted correlation network analysis (WGCNA), and survival analysis identified potential prognostic hub genes. The expression and function of these genes were validated through immunohistochemistry and animal experiments. Specifically, the role of GNAS was investigated through siRNA-mediated knockdown in osteosarcoma cell lines and nude mice models. Results: Five hub genes (PROP1, GNAS, CYP4F2, LHX3, CNGB1) were identified as significantly related to osteosarcoma prognosis. Among these, GNAS was found to be highly expressed in osteosarcoma tissues compared to normal tissues. Immunohistochemical analysis confirmed the elevated expression of GNAS in osteosarcoma samples. GNAS mutation analysis revealed a low mutation rate in osteosarcoma, suggesting its oncogenic role is independent of mutational status. Animal experiments demonstrated that knocking down GNAS significantly inhibited tumor growth and induced apoptosis in osteosarcoma cells. Conclusion: GNAS is highly expressed in osteosarcoma and associated with poor prognosis, acting as an oncogene in osteosarcoma progression. Targeting GNAS could be a potential therapeutic strategy for osteosarcoma. Further studies on GNAS-related signaling pathways may provide deeper insights into the molecular mechanisms driving osteosarcoma malignancy. Competing Interests: The authors have no conflicts of interest to declare. (© Copyright by the Author(s). Published by Cell Physiol Biochem Press.) |
| معلومات مُعتمدة: | YR20220214 China The Talent Introduction Science Foundation of Yijishan Hospital, Wannan Medical College; 2022zhyx-C48 China 2022 Anhui Provincial Institute of Translational Medicine Research Project; WK2021Z06 China The Key Research Foundation of Wannan Medical College |
| فهرسة مساهمة: | Keywords: Gnas ; Osteosarcoma ; Mutation ; Proliferation ; Prognosis |
| المشرفين على المادة: | EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gs) EC 3.6.1.- (GNAS protein, human) 0 (Chromogranins) 0 (RNA, Small Interfering) |
| تواريخ الأحداث: | Date Created: 20240921 Date Completed: 20240921 Latest Revision: 20240921 |
| رمز التحديث: | 20240922 |
| DOI: | 10.33594/000000728 |
| PMID: | 39305132 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1421-9778 |
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| DOI: | 10.33594/000000728 |