دورية أكاديمية
أعرض في EDS

Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids.

التفاصيل البيبلوغرافية
العنوان: Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids.
المؤلفون: Filan C; George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, 30318, USA., Charles S; Georgia Institute of Technology, Interdisciplinary Program in Bioengineering, Atlanta, GA, 30332, USA., Casteleiro Costa P; Georgia Institute of Technology, School of Electrical and Computer Engineering, Atlanta, GA, 30332, USA., Niu W; Department of Psychiatry and Behavioral Sciences, Emory University School of Medicine, Atlanta, Georgia, 30322, USA., Cheng B; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, 30318, USA., Wen Z; Department of Psychiatry and Behavioral Sciences, Emory University School of Medicine, Atlanta, Georgia, 30322, USA.; Departments of Cell Biology and Neurology, Emory University School of Medicine, Atlanta, Georgia, 30322, USA., Lu H; Georgia Institute of Technology, Interdisciplinary Program in Bioengineering, Atlanta, GA, 30332, USA.; Georgia Institute of Technology, School of Chemical and Biomolecular Engineering, Atlanta, Georgia, 30332, USA., Robles FE; George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, 30318, USA. robles@gatech.edu.; Georgia Institute of Technology, Interdisciplinary Program in Bioengineering, Atlanta, GA, 30332, USA. robles@gatech.edu.; Georgia Institute of Technology, School of Electrical and Computer Engineering, Atlanta, GA, 30332, USA. robles@gatech.edu.; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, 30318, USA. robles@gatech.edu.
المصدر: Scientific reports [Sci Rep] 2024 Sep 27; Vol. 14 (1), pp. 22331. Date of Electronic Publication: 2024 Sep 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Organoids*/cytology , Brain*/diagnostic imaging , Brain*/cytology , Imaging, Three-Dimensional*/methods, Humans ; Animals ; Mice ; Cell Culture Techniques, Three Dimensional/methods
مستخلص: Brain organoids provide a unique opportunity to model organ development in a system similar to human organogenesis in vivo. Brain organoids thus hold great promise for drug screening and disease modeling. Conventional approaches to organoid characterization predominantly rely on molecular analysis methods, which are expensive, time-consuming, labor-intensive, and involve the destruction of the valuable three-dimensional (3D) architecture of the organoids. This reliance on end-point assays makes it challenging to assess cellular and subcellular events occurring during organoid development in their 3D context. As a result, the long developmental processes are not monitored nor assessed. The ability to perform non-invasive assays is critical for longitudinally assessing features of organoid development during culture. In this paper, we demonstrate a label-free high-content imaging approach for observing changes in organoid morphology and structural changes occurring at the cellular and subcellular level. Enabled by microfluidic-based culture of 3D cell systems and a novel 3D quantitative phase imaging method, we demonstrate the ability to perform non-destructive high-resolution quantitative image analysis of the organoid. The highlighted results demonstrated in this paper provide a new approach to performing live, non-destructive monitoring of organoid systems during culture.
(© 2024. The Author(s).)
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معلومات مُعتمدة: NSF GRFP DGE-2039655 National Science Foundation Graduate Research Fellowship Program; R21NS117067 United States NS NINDS NIH HHS; R21MH123711 United States MH NIMH NIH HHS; W81XWH1910353 U.S. Department of Defense; CASI BWF 1014540 Burroughs Wellcome Fund; NSF CBET CAREER 356 1752011 National Science Foundation; R35GM147437 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: Brain organoids; Live imaging; Mesofluidics; Neurodevelopmental disorders; Non-invasive imaging; Quantitative phase imaging systems
تواريخ الأحداث: Date Created: 20240927 Date Completed: 20240928 Latest Revision: 20240927
رمز التحديث: 20240928
DOI: 10.1038/s41598-024-72038-2
PMID: 39333572
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-024-72038-2