دورية أكاديمية
Characteristics of an extraction and purification procedure for chlorinated dibenzo-p-dioxins and dibenzofurans in soil and liver.
| العنوان: | Characteristics of an extraction and purification procedure for chlorinated dibenzo-p-dioxins and dibenzofurans in soil and liver. |
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| المؤلفون: | Albro PW, Schroeder JS, Harvan DJ, Corbett BJ |
| المصدر: | Journal of chromatography [J Chromatogr] 1984 Nov 16; Vol. 312, pp. 165-82. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0427043 Publication Model: Print Cited Medium: Print NLM ISO Abbreviation: J Chromatogr Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier. |
| مواضيع طبية MeSH: | Benzofurans/*analysis , Dioxins/*analysis , Liver/*analysis , Polychlorinated Dibenzodioxins/*analysis , Soil/*analysis, Animals ; Chlorine/analysis ; Gas Chromatography-Mass Spectrometry/methods ; Polychlorinated Biphenyls/isolation & purification ; Polychlorinated Dibenzodioxins/analogs & derivatives ; Rats ; Rats, Inbred F344 ; Rats, Inbred Strains ; Solvents |
| مستخلص: | Liver is extracted with chloroform-methanol to give essentially quantitative transfer of endogenous chlorinated dibenzo-p-dioxins (CDDs) and dibenzofurans (CDFs) into the organic phase. A new procedure involving LH-20 Sephadex is used to remove most of the lipids from the extract. Soil is extracted by a simple, rapid and economical procedure giving very high recoveries of CDDs and CDFs from sandy soil, various types of clay, and humus-rich loam. Subsequent cleanup on basic and acidic alumina complete the preparation for gas chromatography-mass spectrometric analysis. The use of propylene glycol as a "keeper" and of 2,3,7-trichlorodibenzo-p-dioxin as a carrier minimizes losses during evaporation of solvents and on glass surfaces. Interactions of 2,3,7,8-CDD with organic material in loam slightly reduce recovery but there is no indication of high affinity binding sites, the losses being apparently associated with simple distribution coefficients. Special precautions needed to avoid losses of CDFs on alumina chromatography are described, and the effect of "aging" spiked soil is discussed. |
| المشرفين على المادة: | 0 (Benzofurans) 0 (Dioxins) 0 (Polychlorinated Dibenzodioxins) 0 (Soil) 0 (Solvents) 4R7X1O2820 (Chlorine) 8U54U639VI (dibenzofuran) DFC2HB4I0K (Polychlorinated Biphenyls) |
| تواريخ الأحداث: | Date Created: 19841116 Date Completed: 19850410 Latest Revision: 20190629 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/s0021-9673(01)92773-6 |
| PMID: | 6441809 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1016/s0021-9673(01)92773-6 |
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