دورية أكاديمية
Histone variants specific to the transcriptionally active, amitotically dividing macronucleus of the unicellular eucaryote, Tetrahymena thermophila.
| العنوان: | Histone variants specific to the transcriptionally active, amitotically dividing macronucleus of the unicellular eucaryote, Tetrahymena thermophila. |
|---|---|
| المؤلفون: | Allis CD, Glover CV, Bowen JK, Gorovsky MA |
| المصدر: | Cell [Cell] 1980 Jul; Vol. 20 (3), pp. 609-17. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print Cited Medium: Print ISSN: 0092-8674 (Print) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, Ma : Cell Press Original Publication: Cambridge, MIT Press. |
| مواضيع طبية MeSH: | Transcription, Genetic*, Histones/*metabolism , Tetrahymena/*ultrastructure, Acetylation ; Animals ; Cell Nucleus/ultrastructure ; Electrophoresis, Polyacrylamide Gel ; Histones/classification ; Peptide Fragments/analysis ; Phosphorylation |
| مستخلص: | Two dimensional gel electrophoresis (triton-acid-urea followed by SDS) has been used to resolve two previously uncharacterized, quantitatively minor histone variants in acid extracts from macronuclei of Tetrahymena thermophila. Utilizing techniques which allow characterization of these variants without purifying them in significant quantities, we identify one protein as a subtype of H3. The other protein is a moderately lysine-rich histone whose tryptic peptide map differs from that of both H2A and H2B. However, its pattern of secondary modifications, its detergent-binding properties and its methionineless nature all suggest that it is more like H2A than any other histone. Both variants are associated with nucleosomes derived from macronuclei. Thus primary sequence variants of the inner histones, presumably indicative of nucleosome heterogeneity, exist in a lower eucaryote, in an amitotic nucleus, and within the nucleus of a clonally propagated organism. Evidence is presented that these newly described minor variants are absent in micronuclei, suggesting that they play an important role in the structural and functional differentiation of macronuclear chromatin. |
| المشرفين على المادة: | 0 (Histones) 0 (Peptide Fragments) |
| تواريخ الأحداث: | Date Created: 19800701 Date Completed: 19801218 Latest Revision: 20190705 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/0092-8674(80)90307-4 |
| PMID: | 7418000 |
| قاعدة البيانات: | MEDLINE |
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