دورية أكاديمية
أعرض في EDS

Transgene inactivation in Petunia hybrida is influenced by the properties of the foreign gene.

التفاصيل البيبلوغرافية
العنوان: Transgene inactivation in Petunia hybrida is influenced by the properties of the foreign gene.
المؤلفون: Elomaa P; Institute of Biotechnology, University of Helsinki, Finland., Helariutta Y, Griesbach RJ, Kotilainen M, Seppänen P, Teeri TH
المصدر: Molecular & general genetics : MGG [Mol Gen Genet] 1995 Oct 25; Vol. 248 (6), pp. 649-56.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0125036 Publication Model: Print Cited Medium: Print ISSN: 0026-8925 (Print) Linking ISSN: 00268925 NLM ISO Abbreviation: Mol Gen Genet Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin ; New York Springer-Verlag [1967-
مواضيع طبية MeSH: Gene Expression Regulation, Plant* , Transgenes*, Pigmentation/*genetics , Plants, Genetically Modified/*genetics , Transformation, Genetic/*genetics, Alcohol Oxidoreductases/genetics ; Anthocyanins/analysis ; Anthocyanins/chemistry ; Blotting, Northern ; Blotting, Southern ; DNA, Complementary/genetics ; Flavonoids/biosynthesis ; Flavonoids/genetics ; Gene Dosage ; Genetic Vectors ; Methylation ; Mutation/genetics ; Phenotype ; Pigments, Biological/biosynthesis ; Pigments, Biological/genetics ; Promoter Regions, Genetic/genetics ; Zea mays/genetics
مستخلص: Petunia mutant RL01 was transformed with maize A1 and gerbera gdfr cDNAs, which both encode dihydroflavonol-4-reductase (DFR) activity. The same Agrobacterium vector and the same version of the CaMV 35S promoter were used in both experiments. Transformation with the cDNAs resulted in production of pelargonidin pigments in the transformants. However, the A1 and gdfr transformants showed clearly different phenotypes. The flowers of the primary A1 transformants were pale and showed variability in pigmentation during their growth, while the flowers of the gdfr transformants showed intense and highly stable coloration. The color difference in the primary transformants was reflected in the expression levels of the transgenes as well as in the levels of anthocyanin pigment. As previously reported by others, the instability in pigmentation in the A1 transformants was more often detected in clones with multiple copies of the transgene and was associated with methylation of the 35S promoter and of the transgene cDNA itself. In the gdfr transformants, the most intense pigmentation was observed in plants with multiple transgenes in their genome. Only rarely was partial methylation of the 35S promoter detected, while the gdfr cDNA always remained in an unmethylated state. We conclude that the properties of the transgene itself strongly influence the inactivation process. The dicotyledonous gdfr cDNA with a lower GC content and fewer possible methylation sites is more 'compatible' the genomic organization of petunia and this prevents it being recognized as a foreign gene and hence silenced by methylation.
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المشرفين على المادة: 0 (Anthocyanins)
0 (DNA, Complementary)
0 (Flavonoids)
0 (Pigments, Biological)
7690-51-9 (pelargonidin)
EC 1.1.- (Alcohol Oxidoreductases)
EC 1.1.1.- (dihydroflavanol 4-reductase)
تواريخ الأحداث: Date Created: 19951025 Date Completed: 19951226 Latest Revision: 20220408
رمز التحديث: 20221213
DOI: 10.1007/BF02191704
PMID: 7476867
قاعدة البيانات: MEDLINE
الوصف
تدمد:0026-8925
DOI:10.1007/BF02191704