دورية أكاديمية
Generation of the precursor (lyso-PAF) of platelet-activating factor via a CoA-dependent transacylase.
| العنوان: | Generation of the precursor (lyso-PAF) of platelet-activating factor via a CoA-dependent transacylase. |
|---|---|
| المؤلفون: | Blank ML; Medical Sciences Division, Oak Ridge Associated Universities, Tennessee 37831-0117, USA., Fitzgerald V, Smith ZL, Snyder F |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1995 May 25; Vol. 210 (3), pp. 1052-8. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print Cited Medium: Print ISSN: 0006-291X (Print) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | 1-Acylglycerophosphocholine O-Acyltransferase/*metabolism , Acyltransferases/*metabolism , Microsomes/*enzymology , Platelet Activating Factor/*analogs & derivatives , Spleen/*enzymology, Adenosine Triphosphate/metabolism ; Animals ; Coenzyme A/pharmacology ; Kinetics ; Male ; Platelet Activating Factor/biosynthesis ; Rats ; Rats, Inbred Strains ; Serum Albumin, Bovine/pharmacology ; Substrate Specificity |
| مستخلص: | The microsomal fraction from rat spleen was shown to possess a CoA-dependent transacylase activity that produced 1-[3H]alkyl-2-lyso-sn-glycero-3-phosphocholine ([3H]lyso-PAF), the immediate precursor of PAF in the remodeling pathway of biosynthesis, from 1-[3H]alkyl-2-acyl-sn-glycero-3-phosphocholine. This CoA-dependent transacylase did not require ATP or metal ions for activity making it unlikely that either acyl-CoA-synthetase or a Ca(2+)-dependent phospholipase A2 were involved in the generation of [3H]lyso-PAF. Albumin, in addition to CoA, was required to demonstrate the formation of [3H]lyso-PAF from 1-[3H]alkyl-2-acyl-sn-glycero-3-phosphocholine. It appeared that a major function of albumin in the incubations was to complex the [3H]lyso-PAF formed, thus removing this end-product from the reaction. |
| المشرفين على المادة: | 0 (O-deacetyl platelet activating factor) 0 (Platelet Activating Factor) 27432CM55Q (Serum Albumin, Bovine) 8L70Q75FXE (Adenosine Triphosphate) EC 2.3.- (Acyltransferases) EC 2.3.1.- (arachidonyl transacylase) EC 2.3.1.23 (1-Acylglycerophosphocholine O-Acyltransferase) SAA04E81UX (Coenzyme A) |
| تواريخ الأحداث: | Date Created: 19950525 Date Completed: 19950629 Latest Revision: 20171116 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1006/bbrc.1995.1763 |
| PMID: | 7763233 |
| قاعدة البيانات: | MEDLINE |
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