دورية أكاديمية
Phospholipid/alkanethiol bilayers for cell-surface receptor studies by surface plasmon resonance.
| العنوان: | Phospholipid/alkanethiol bilayers for cell-surface receptor studies by surface plasmon resonance. |
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| المؤلفون: | Plant AL; Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA., Brigham-Burke M, Petrella EC, O'Shannessy DJ |
| المصدر: | Analytical biochemistry [Anal Biochem] 1995 Apr 10; Vol. 226 (2), pp. 342-8. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0370535 Publication Model: Print Cited Medium: Print ISSN: 0003-2697 (Print) Linking ISSN: 00032697 NLM ISO Abbreviation: Anal Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : San Diego, CA : Elsevier Original Publication: Orlando Fl : Academic Press |
| مواضيع طبية MeSH: | Biosensing Techniques*, Lipid Bilayers/*metabolism , Receptors, Cell Surface/*metabolism, Biotin/metabolism ; Electric Impedance ; Gold/chemistry ; Immunoglobulin G/metabolism ; Kinetics ; Ligands ; Liposomes/metabolism ; Phospholipids/chemistry ; Phospholipids/metabolism ; Receptors, Cell Surface/analysis ; Serum Albumin, Bovine/metabolism ; Sulfhydryl Compounds/chemistry ; Wheat Germ Agglutinins/metabolism |
| مستخلص: | Supported hybrid bilayer membranes (HBM) composed of a monolayer of phospholipid and a monolayer of alkanethiol associated with a thin gold film on glass are useful as model lipid bilayer membranes for studying membrane receptor-ligand and cell-cell binding events by surface plasmon resonance (SPR). Measurements of specific binding of proteins and lipid vesicles to well-defined HBMs have been performed under conditions of continuous flow using a commercial SPR instrument (BIAcore). HBMs are shown to be stable in flow and to block nonspecific adsorption of proteins to the alkanethiol/gold surface. The use of such supported lipid bilayers in flow provides a means of conducting equilibrium and kinetic studies of models of ligand-cell and cell-cell interactions with receptors or ligands in a membrane environment. Compared to the extended dextran polymer layer that is currently used for surface modification of BIAcore "sensor chips," the described HBMs provide a well-defined surface that will permit less ambiguous modeling of these important biological interactions. |
| المشرفين على المادة: | 0 (Immunoglobulin G) 0 (Ligands) 0 (Lipid Bilayers) 0 (Liposomes) 0 (Phospholipids) 0 (Receptors, Cell Surface) 0 (Sulfhydryl Compounds) 0 (Wheat Germ Agglutinins) 27432CM55Q (Serum Albumin, Bovine) 6SO6U10H04 (Biotin) 7440-57-5 (Gold) |
| تواريخ الأحداث: | Date Created: 19950410 Date Completed: 19950727 Latest Revision: 20190628 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1006/abio.1995.1234 |
| PMID: | 7793636 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0003-2697 |
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| DOI: | 10.1006/abio.1995.1234 |