دورية أكاديمية
Opposite functions of jun-B and c-jun in growth regulation and neuronal differentiation.
| العنوان: | Opposite functions of jun-B and c-jun in growth regulation and neuronal differentiation. |
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| المؤلفون: | Schlingensiepen KH; Department of Neurobiology, Max-Planck-Institut für Biophysikalische Chemie, Germany., Schlingensiepen R, Kunst M, Klinger I, Gerdes W, Seifert W, Brysch W |
| المصدر: | Developmental genetics [Dev Genet] 1993; Vol. 14 (4), pp. 305-12. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 7909963 Publication Model: Print Cited Medium: Print ISSN: 0192-253X (Print) Linking ISSN: 0192253X NLM ISO Abbreviation: Dev Genet Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York Ny : Wiley-Liss Original Publication: New York, Liss. |
| مواضيع طبية MeSH: | Gene Expression Regulation*/drug effects, Neurons/*cytology , Oligonucleotides, Antisense/*pharmacology , Proto-Oncogene Proteins c-jun/*physiology , Thionucleotides/*pharmacology, 3T3 Cells ; Adrenal Gland Neoplasms/pathology ; Animals ; Base Sequence ; Breast Neoplasms/pathology ; Cell Differentiation/genetics ; Cell Division/genetics ; Mice ; Molecular Sequence Data ; Nerve Growth Factors/pharmacology ; Neurons/metabolism ; Pheochromocytoma/pathology ; Proto-Oncogene Proteins c-jun/genetics ; Transcriptional Activation ; Tumor Cells, Cultured |
| مستخلص: | Induction of the jun-B and/or c-jun transcription factors is part of the immediate early response to diverse stimuli that induce alterations in cellular programs. While c-jun is a protooncogene whose expression is required for induction of cell proliferation, jun-B has recently been found to be induced by stimuli inducing differentiation in various cell lines. Furthermore, its expression is largely restricted to differentiating cells during embryogenesis. To determine the functional significance of these findings, we used antisense phosphorothioate oligodeoxynucleotides to inhibit expression of the two genes in proliferating and neuronally differentiating cells. While selective inhibition of c-jun expression reduced proliferation rates, inhibition of jun-B protein synthesis markedly increased proliferation in 3T3 fibroblasts, human mammary carcinoma cells and PC-12 pheochromocytoma cells, suggesting jun-B involvement in negative growth control. Neuronal differentiation of PC-12 cells induced by nerve growth factor (NGF) was prevented by inhibition of jun-B protein synthesis. PC-12 cells not only failed to grow neurites but also remained in the proliferative state. Furthermore, in cultured primary neurons from rat hippocampus, inhibition of jun-B expression, again, markedly reduced morphological differentiation. Conversely, inhibition of c-jun protein synthesis enhanced morphological differentiation of both primary neurons and PC-12 tumor cells. Thus, jun-B expression is required for neuronal differentiation and its balance with c-jun activity is involved in regulating key steps in proliferation and differentiation processes. |
| Gene Symbol: | c-jun; c-myb; jun-B; ras |
| المشرفين على المادة: | 0 (Nerve Growth Factors) 0 (Oligonucleotides, Antisense) 0 (Proto-Oncogene Proteins c-jun) 0 (Thionucleotides) |
| تواريخ الأحداث: | Date Created: 19930101 Date Completed: 19931207 Latest Revision: 20081121 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1002/dvg.1020140408 |
| PMID: | 8222345 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0192-253X |
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| DOI: | 10.1002/dvg.1020140408 |