دورية أكاديمية
Adaptive changes in the number of Gs- and Gi-proteins underlie adenylyl cyclase sensitization in morphine-treated rat striatal neurons.
| العنوان: | Adaptive changes in the number of Gs- and Gi-proteins underlie adenylyl cyclase sensitization in morphine-treated rat striatal neurons. |
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| المؤلفون: | Van Vliet BJ; Department of Pharmacology, Free University, Medical Faculty, Amsterdam, Netherlands., Van Rijswijk AL, Wardeh G, Mulder AH, Schoffelmeer AN |
| المصدر: | European journal of pharmacology [Eur J Pharmacol] 1993 Mar 15; Vol. 245 (1), pp. 23-9. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 1254354 Publication Model: Print Cited Medium: Print ISSN: 0014-2999 (Print) Linking ISSN: 00142999 NLM ISO Abbreviation: Eur J Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2005- : Amsterdam : Elsevier Science Original Publication: Amsterdam, North Holland Pub. Co. |
| مواضيع طبية MeSH: | Adenylyl Cyclases/*metabolism , Corpus Striatum/*drug effects , GTP-Binding Proteins/*metabolism , Morphine/*pharmacology , Receptors, Dopamine D1/*metabolism, 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine/pharmacology ; Analysis of Variance ; Animals ; Cells, Cultured ; Corpus Striatum/cytology ; Corpus Striatum/enzymology ; Cyclic AMP/metabolism ; Drug Tolerance ; Mice ; Morphine Dependence ; Radioligand Assay ; Rats ; Rats, Wistar ; Receptors, Dopamine D1/drug effects |
| مستخلص: | In the present study we investigated the possible role of changes in the number of membrane-bound G-proteins in the sensitization of dopamine D1 receptor-stimulated adenylyl cyclase, observed in primary cultures of rat striatal neurons chronically exposed to morphine. Whereas exposure of these neurons to 10 microM morphine for 7 days caused a profound increase in cyclic AMP production, induced by the dopamine D1 receptor agonist SKF 38393 (1 microM), Scatchard analysis of [125I]SCH 23982 binding to membrane preparations revealed that neither the Bmax nor the Kd values of dopamine D1 receptor binding sites were affected. Interestingly, immunoblotting experiments revealed an increase (of more than 50%) in the number of stimulatory G-proteins (G alpha s) in neurons displaying an enhanced adenylyl cyclase activity. In morphine-treated neurons, the number of inhibitory G-proteins (G alpha i) appeared to be slightly reduced (by about 16%). Moreover, the observation that cholera toxin (0.1 nM)-stimulated cyclic AMP production, unlike that induced by forskolin (1 microM), was enhanced in morphine-treated neurons, indicates a causal relationship between the reciprocal changes in G-protein number and the increase of dopamine D1 receptor-stimulated adenylyl cyclase activity. The possible role of these changes in G-protein number in the development of morphine tolerance and dependence is discussed. |
| المشرفين على المادة: | 0 (Receptors, Dopamine D1) 67287-49-4 (2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine) 76I7G6D29C (Morphine) E0399OZS9N (Cyclic AMP) EC 3.6.1.- (GTP-Binding Proteins) EC 4.6.1.1 (Adenylyl Cyclases) |
| تواريخ الأحداث: | Date Created: 19930315 Date Completed: 19930521 Latest Revision: 20190624 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/0922-4106(93)90165-6 |
| PMID: | 8386668 |
| قاعدة البيانات: | MEDLINE |
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