دورية أكاديمية
أعرض في EDS

Expression of a protective gene-prolongs survival of T cells in human immunodeficiency virus-infected patients.

التفاصيل البيبلوغرافية
العنوان: Expression of a protective gene-prolongs survival of T cells in human immunodeficiency virus-infected patients.
المؤلفون: Woffendin C; Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109-0650, USA., Ranga U, Yang Z, Xu L, Nabel GJ
المصدر: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1996 Apr 02; Vol. 93 (7), pp. 2889-94.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, P.H.S.
اللغة: English
بيانات الدورية: Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Print ISSN: 0027-8424 (Print) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : National Academy of Sciences
مواضيع طبية MeSH: Genes, rev* , Genetic Therapy*, CD4-Positive T-Lymphocytes/*physiology , Gene Products, rev/*biosynthesis , HIV Infections/*immunology , HIV Infections/*therapy , T-Lymphocytes/*physiology, Acquired Immunodeficiency Syndrome/immunology ; Acquired Immunodeficiency Syndrome/mortality ; Acquired Immunodeficiency Syndrome/therapy ; Base Sequence ; CD4 Lymphocyte Count ; CD4-Positive T-Lymphocytes/pathology ; Cell Survival ; DNA Primers ; Gene Products, rev/genetics ; Gene Transfer Techniques ; Genetic Vectors ; HIV Infections/mortality ; HIV-1 ; Humans ; Lymphocyte Transfusion ; Male ; Molecular Sequence Data ; Polymerase Chain Reaction ; Sequence Deletion ; Survival Rate ; T-Lymphocytes/pathology ; rev Gene Products, Human Immunodeficiency Virus
مستخلص: The resistance of acquired immunodeficiency syndrome (AIDS) to traditional drug therapy has prompted a search for alternative treatments for this disease. One potential approach is to provide genetic resistance to viral replication to prolong latency. This strategy requires the definition of effective antiviral genes that extend the survival of T cells in human immunodeficiency virus (HIV)-infected individuals. We report the results of a human study designed to determine whether a genetic intervention can prolong the survival of T cells in HIV-infected individuals. Gene transfer was performed in enriched CD4+ cells with plasmid expression vectors encoding an inhibitory Rev protein, Rev M10, or a deletion mutant control, deltaRev M10, delivered by gold microparticles. Autologous cells separately transfected with each of the vectors were returned to each patient, and toxicity, gene expression, and survival of genetically modified cells were assessed. Cells that expressed Rev M10 were more resistant to HIV infection than those with deltaRev M10 in vitro. In HIV-infected subjects, Rev M10-transduced cells showed preferential survival compared to deltaRev M10 controls. Rev M10 can therefore act as a specific intracellular inhibitor that can prolong T-cell survival in HIV-1-infected individuals and potentially serve as a molecular genetic intervention which can contribute to the treatment of AIDS.
References: Nature. 1987 Sep 17-23;329(6136):219-22. (PMID: 2442619)
Gene Ther. 1994 Jan;1(1):32-7. (PMID: 7584057)
Nature. 1988 Sep 22;335(6188):369-72. (PMID: 2843774)
Nature. 1988 Sep 29;335(6189):395-6. (PMID: 3166513)
Nature. 1988 Sep 29;335(6189):452-4. (PMID: 2843776)
Cell. 1989 Jul 14;58(1):205-14. (PMID: 2752419)
Cell. 1989 Oct 6;59(1):113-20. (PMID: 2676192)
Science. 1990 Mar 9;247(4947):1222-5. (PMID: 2107573)
Cell. 1990 Jun 29;61(7):1271-6. (PMID: 2364429)
Proc Natl Acad Sci U S A. 1990 Jul;87(13):5079-83. (PMID: 2195547)
Cell. 1990 Nov 2;63(3):601-8. (PMID: 2225067)
J Virol. 1991 Aug;65(8):4248-54. (PMID: 2072452)
Annu Rev Biochem. 1991;60:577-630. (PMID: 1883204)
J Exp Med. 1992 Oct 1;176(4):1197-201. (PMID: 1402661)
Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9870-4. (PMID: 1409715)
Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10802-6. (PMID: 1438280)
Science. 1992 Nov 27;258(5087):1485-8. (PMID: 1359646)
J Virol. 1993 Jun;67(6):3199-207. (PMID: 8388497)
Antimicrob Agents Chemother. 1993 May;37(5):1127-31. (PMID: 7685995)
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6340-4. (PMID: 8327516)
Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7889-93. (PMID: 8356098)
Nucleic Acids Res. 1993 Aug 11;21(16):3917-8. (PMID: 8367319)
Hum Gene Ther. 1994 Jan;5(1):79-92. (PMID: 8155773)
Proc Natl Acad Sci U S A. 1994 May 24;91(11):5075-9. (PMID: 8197188)
Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11581-5. (PMID: 7972106)
Nature. 1995 Jan 12;373(6510):117-22. (PMID: 7529365)
Nature. 1995 Jan 12;373(6510):123-6. (PMID: 7816094)
Science. 1995 Jan 27;267(5197):483-9. (PMID: 7824947)
N Engl J Med. 1995 Mar 2;332(9):567-75. (PMID: 7646637)
Hum Gene Ther. 1995 Aug;6(8):997-1004. (PMID: 7578421)
Gene Ther. 1994 Jan;1(1):13-26. (PMID: 7584055)
J Virol. 1988 Jul;62(7):2498-501. (PMID: 2836628)
معلومات مُعتمدة: 1U01-AI33355-01 United States AI NIAID NIH HHS; 1U19AI36606-01 United States AI NIAID NIH HHS; M01-RR00042 United States RR NCRR NIH HHS
المشرفين على المادة: 0 (DNA Primers)
0 (Gene Products, rev)
0 (rev Gene Products, Human Immunodeficiency Virus)
تواريخ الأحداث: Date Created: 19960402 Date Completed: 19960524 Latest Revision: 20190501
رمز التحديث: 20240627
مُعرف محوري في PubMed: PMC39729
DOI: 10.1073/pnas.93.7.2889
PMID: 8610137
قاعدة البيانات: MEDLINE
الوصف
تدمد:0027-8424
DOI:10.1073/pnas.93.7.2889