دورية أكاديمية
Characterization of an interleukin 6 cytokine family antagonist protein from a marine sponge, Callyspongia sp.
| العنوان: | Characterization of an interleukin 6 cytokine family antagonist protein from a marine sponge, Callyspongia sp. |
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| المؤلفون: | Peppard JV; Pharmaceuticals Division, Ciba-Geigy Corporation, Summit, New Jersey 07901, USA., Loo P, Sills MA, Munster D, Pomponi SA, Wright AE |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 1996 Mar 29; Vol. 271 (13), pp. 7281-4. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print Cited Medium: Print ISSN: 0021-9258 (Print) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Antigens, CD/*chemistry , Interleukin-6/*antagonists & inhibitors , Interleukin-6/*pharmacology , Membrane Glycoproteins/*isolation & purification , Membrane Glycoproteins/*pharmacology , Porifera/*chemistry , Receptors, Interleukin/*chemistry, Animals ; Carcinoma, Hepatocellular ; Cell Line ; Cytokines/antagonists & inhibitors ; Haptoglobins/biosynthesis ; Interleukin-6/metabolism ; Kinetics ; Liver Neoplasms ; Receptors, Interleukin-6 ; Signal Transduction ; Tumor Cells, Cultured |
| مستخلص: | An inhibitor of IL-6 binding to the human hepatoma line HepG2 and myeloma cell line U266 was identified in a saline extract of the marine sponge, Callyspongia sp. Functional activity, measured through the increase in haptoglobin production by HepG2 cells stimulated with IL-6, could be strongly inhibited by the extract. Similarly, IL-6-induced production of IgM by the B cell line SKW6.4 was substantially reduced. In neither cell line was there evidence of toxicity produced by the extract. Other sponges of the Callyspongia species were found to contain analogous activity. The activity was destroyed by trypsin treatment or boiling of the extract, suggesting that the inhibition is due to a protein. When the binding of IL-6 to its receptor complex was dissected in vitro, inhibition of binding of IL-6 to soluble receptor by the extract was not detected, but binding of the IL-6-sIL-6R complex to soluble gp130 was inhibited in a dose-dependent fashion. This was borne out in cellular assays since the extract inhibited activation of HepG2 cells stimulated with oncostatin M or leukemia inhibitory factor, cytokines which also use gp130 for signal transduction. These results suggest that the Callyspongia extract contains a protein which blocks the interaction of the IL-6 family of cytokines with their signal transduction moiety, gp130. Elucidation of the structure and mode of action of such a protein would be helpful in designing gp130 antagonists to inhibit the functions of this cytokine family, overproduction of which has been associated with cancer and pathologies of autoimmune disease and AIDS. |
| المشرفين على المادة: | 0 (Antigens, CD) 0 (Cytokines) 0 (Haptoglobins) 0 (Interleukin-6) 0 (Membrane Glycoproteins) 0 (Receptors, Interleukin) 0 (Receptors, Interleukin-6) |
| تواريخ الأحداث: | Date Created: 19960329 Date Completed: 19960703 Latest Revision: 20210210 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1074/jbc.271.13.7281 |
| PMID: | 8631742 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0021-9258 |
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| DOI: | 10.1074/jbc.271.13.7281 |