دورية أكاديمية
A large variety of alternatively spliced and differentially expressed mRNAs are encoded by the human acute myeloid leukemia gene AML1.
| العنوان: | A large variety of alternatively spliced and differentially expressed mRNAs are encoded by the human acute myeloid leukemia gene AML1. |
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| المؤلفون: | Levanon D; Department of Molecular Genetics and Virology, The Weizmann Institite of Science, Rehovot, Israel., Bernstein Y, Negreanu V, Ghozi MC, Bar-Am I, Aloya R, Goldenberg D, Lotem J, Groner Y |
| المصدر: | DNA and cell biology [DNA Cell Biol] 1996 Mar; Vol. 15 (3), pp. 175-85. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert Country of Publication: United States NLM ID: 9004522 Publication Model: Print Cited Medium: Print ISSN: 1044-5498 (Print) Linking ISSN: 10445498 NLM ISO Abbreviation: DNA Cell Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Larchmont, NY : Mary Ann Liebert Original Publication: [New York, NY] : Mary Ann Liebert, [c1990- |
| مواضيع طبية MeSH: | DNA-Binding Proteins* , Proto-Oncogene Proteins*, Neoplasm Proteins/*genetics , Transcription Factors/*genetics, Alternative Splicing ; Animals ; Base Sequence ; Core Binding Factor Alpha 2 Subunit ; DNA Primers/chemistry ; DNA, Complementary/genetics ; Gene Expression Regulation, Neoplastic ; Genes ; Humans ; In Situ Hybridization ; Mice ; Molecular Sequence Data ; Nucleic Acid Conformation ; RNA, Neoplasm/genetics ; Thymus Gland |
| مستخلص: | The human chromosome 21 acute myeloid leukemia gene AML1 is frequently rearranged in the leukemia-associated translocations t(8;21) and t(3;21), generating fused proteins containing the amino-terminal part of AML1. In normal blood cells, five size classes (2-8 kb) of AML1 mRNAs have been previously observed. We isolated seven cDNAs corresponding to various AML1 mRNAs. Sequencing revealed that their size differences were mainly due to alternatively spliced 5' and 3' untranslated regions, some of which were vast, exceeding 1.5 kb (5') and 4.3 kb (3'). These untranslated regions contain sequences known to control mRNA translation and stability and seem to modulate AML1 mRNA stability. Further heterogeneity was found in the coding region due to the presence of alternatively spliced stop codon-containing exons. The latter led to production of polypeptides that were smaller than the full-length AML1 protein; they lacked the trans-activation domains but maintained DNA binding and heterodimerization ability. The size of these truncated products was similar to the AML1 segment in the fused t(8;21) and t(3;21) proteins. In thymus, only one mRNA species of 6 kb was detected. Using in situ hybridization, we showed that its expression was confined to the cortical region of the organ. The 6-kb mRNA was also prominent in cultured peripheral blood T cells, and its expression was markedly reduced upon mitogenic activation by phorbol myristate acetate (TPA) plus concanavalin A (ConA). These results and the presence of multiple coding regions flanked by long complex untranslated regions, suggest that AML1 expression is regulated at different levels by several control mechanisms generating the large variety of mRNAs and protein products. |
| سلسلة جزيئية: | GENBANK AJ229043; X90976; X90977; X90978; X90979; X90980; X90981 |
| المشرفين على المادة: | 0 (Core Binding Factor Alpha 2 Subunit) 0 (DNA Primers) 0 (DNA, Complementary) 0 (DNA-Binding Proteins) 0 (Neoplasm Proteins) 0 (Proto-Oncogene Proteins) 0 (RNA, Neoplasm) 0 (RUNX1 protein, human) 0 (Runx1 protein, mouse) 0 (Transcription Factors) |
| تواريخ الأحداث: | Date Created: 19960301 Date Completed: 19960709 Latest Revision: 20061115 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1089/dna.1996.15.175 |
| PMID: | 8634147 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1044-5498 |
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| DOI: | 10.1089/dna.1996.15.175 |