دورية أكاديمية
Inhibition of pp125FAK in cultured fibroblasts results in apoptosis.
| العنوان: | Inhibition of pp125FAK in cultured fibroblasts results in apoptosis. |
|---|---|
| المؤلفون: | Hungerford JE; Department of Cell Biology, School of Medicine, University of Virginia, Charlottesville 22908, USA., Compton MT, Matter ML, Hoffstrom BG, Otey CA |
| المصدر: | The Journal of cell biology [J Cell Biol] 1996 Dec; Vol. 135 (5), pp. 1383-90. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print Cited Medium: Print ISSN: 0021-9525 (Print) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York : Rockefeller University Press |
| مواضيع طبية MeSH: | Apoptosis*, Cell Adhesion Molecules/*metabolism , Protein-Tyrosine Kinases/*metabolism, Amino Acid Sequence ; Animals ; Binding Sites ; Binding, Competitive ; Cell Adhesion Molecules/immunology ; Cell Adhesion Molecules/pharmacology ; Cell Membrane/ultrastructure ; Cell Nucleus/ultrastructure ; Cells, Cultured ; Chick Embryo ; Enzyme Activation ; Fibroblasts ; Focal Adhesion Kinase 1 ; Focal Adhesion Protein-Tyrosine Kinases ; Integrin beta1/chemistry ; Integrin beta1/metabolism ; Molecular Sequence Data ; Peptides/chemistry ; Peptides/metabolism ; Protein-Tyrosine Kinases/immunology ; Protein-Tyrosine Kinases/pharmacology ; Signal Transduction |
| مستخلص: | The tyrosine kinase called pp125FAK is believed to play an important role in integrin-mediated signal transduction. pp125FAK is associated both functionally and spatially with integrins, which are the cell surface receptors for extracellular matrix components. Although the precise function of pp125FAK is not known, two possibilities have been proposed: pp125FAK may regulate the assembly of focal adhesions in spreading or migrating cells, or pp125FAK may participate in a signal transduction cascade to inform the nucleus that the cell is anchored. To test these models in living cells, a peptide representing the focal adhesion kinase (FAK)-binding site of the beta 1 tail was coupled to carrier protein and injected into cultured cells to competitively inhibit the binding of pp125FAK to endogenous integrin, thus inhibiting activation of pp125FAK on a cell-by-cell basis. In addition, an antibody directed against an epitope adjacent to the focal adhesion targeting sequence on pp125FAK was microinjected, as an alternative means of inhibiting pp125FAK activation. It was observed that when rounded cells were injected with either the integrin peptide or the anti-FAK antibody, the cells rapidly began to apoptose, within 4 h after injection. These results indicate that pp125FAK may play a critical role in suppressing apoptosis in fibroblasts. |
| References: | Mol Biol Cell. 1995 Nov;6(11):1443-58. (PMID: 8589448) Cell. 1994 May 20;77(4):477-8. (PMID: 8187171) J Cell Sci. 1995 Jun;108 ( Pt 6):2381-91. (PMID: 7673357) J Cell Biol. 1992 Nov;119(4):893-903. (PMID: 1385444) J Cell Biol. 1995 Nov;131(3):791-805. (PMID: 7593197) J Biol Chem. 1992 Nov 25;267(33):23439-42. (PMID: 1429685) Science. 1995 Feb 10;267(5199):891-3. (PMID: 7531366) J Cell Sci. 1996 Mar;109 ( Pt 3):631-42. (PMID: 8907708) Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5192-6. (PMID: 1594631) J Cell Sci. 1994 May;107 ( Pt 5):1169-79. (PMID: 7929626) Curr Opin Cell Biol. 1993 Oct;5(5):819-31. (PMID: 8240826) Nature. 1992 Aug 20;358(6388):690-2. (PMID: 1379699) Cell. 1994 Dec 30;79(7):1157-64. (PMID: 7528107) Science. 1995 Apr 14;268(5208):233-9. (PMID: 7716514) Nature. 1996 Apr 11;380(6574):538-40. (PMID: 8606775) J Biol Chem. 1993 Oct 5;268(28):21193-7. (PMID: 7691808) Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8392-6. (PMID: 1717976) Cell Growth Differ. 1996 Apr;7(4):413-8. (PMID: 9052982) Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8487-91. (PMID: 1528852) Cell Regul. 1991 Nov;2(11):951-64. (PMID: 1725602) Nature. 1995 Oct 12;377(6549):539-44. (PMID: 7566154) J Cell Biol. 1994 Feb;124(4):619-26. (PMID: 8106557) J Cell Biol. 1996 Aug;134(3):793-9. (PMID: 8707856) Mol Biol Cell. 1993 Sep;4(9):953-61. (PMID: 8257797) J Cell Biol. 1994 Oct;127(2):537-46. (PMID: 7523422) Cell. 1995 Oct 20;83(2):279-87. (PMID: 7585945) Trends Cell Biol. 1992 Oct;2(10):304-8. (PMID: 14731926) Int Rev Cytol. 1996;167:161-83. (PMID: 8768494) Curr Opin Cell Biol. 1992 Oct;4(5):772-81. (PMID: 1329869) J Cell Biol. 1993 Feb;120(3):577-85. (PMID: 8381117) EMBO J. 1995 Nov 1;14(21):5179-90. (PMID: 7489707) J Cell Biol. 1995 Sep;130(5):1181-7. (PMID: 7657702) Mol Biol Cell. 1996 Aug;7(8):1209-24. (PMID: 8856665) |
| معلومات مُعتمدة: | GM50974 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (Cell Adhesion Molecules) 0 (Integrin beta1) 0 (Peptides) EC 2.7.10.1 (Protein-Tyrosine Kinases) EC 2.7.10.2 (Focal Adhesion Kinase 1) EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases) |
| تواريخ الأحداث: | Date Created: 19961201 Date Completed: 19970102 Latest Revision: 20220310 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC2121083 |
| DOI: | 10.1083/jcb.135.5.1383 |
| PMID: | 8947559 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0021-9525 |
|---|---|
| DOI: | 10.1083/jcb.135.5.1383 |