دورية أكاديمية
A rapid stain-clearing method for video based cytological analysis of cotton megagametophytes.
| العنوان: | A rapid stain-clearing method for video based cytological analysis of cotton megagametophytes. |
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| المؤلفون: | Hodnett GL; Department of Soil and Crop Sciences, Texas A & M University, College Station 77843-2474, USA., Crane CF, Stelly DM |
| المصدر: | Biotechnic & histochemistry : official publication of the Biological Stain Commission [Biotech Histochem] 1997 Jan; Vol. 72 (1), pp. 16-21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9107378 Publication Model: Print Cited Medium: Print ISSN: 1052-0295 (Print) Linking ISSN: 10520295 NLM ISO Abbreviation: Biotech Histochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Abingdon, Oxford : Taylor & Francis Original Publication: Baltimore; MD : Williams & Wilkins, c1991- |
| مواضيع طبية MeSH: | Gossypium/*cytology , Microscopy, Video/*methods, Coloring Agents |
| مستخلص: | Optical "clearing" is a cost saving method for preparing large numbers of whole, dissected or thickly sectioned cytological specimens such as plant ovules and ovaries. Minimal labor is required and specimens retain three-dimensional integrity. Previous development of high contrast stain-clearing methods using hemalum to impart contrast has facilitated analysis and photography under bright field illumination for small ovules. The deep stain intensity of hemalum, however, often precludes adequate light transmission and contrast within internal focal planes, limiting the applicability of hemalum-based stain-clearing to small specimens. Having encountered this problem for nucelli of cotton (Gossypium barbadense L.), which are roughly 300 microns thick at fertilization, we have developed a modified stain-clearing system. The two key features of these new methods are the use of azure, C, which allows the intensity of staining to be readily regulated, and contrast manipulation via video signal and image processing. Intensity of azure C stain was readily controlled by modifying the staining and/or dehydration media to produce relatively low contrast specimens. Analysis was facilitated by indirect viewing on a video monitor using adjustments of sensitivity, exposure, and contrast of the charge-coupled device (CCD) camera. Digital processing provided further enhancement. Acceptable images were obtained from virtually all specimens. These methods, which combine low contrast (high transmittance) specimens with high contrast imaging, should facilitate data acquisition on reproduction, thus the developmental and genetic characterization of reproductive mutants. Other applications, e.g., in pathology and embryology, are readily envisioned. |
| المشرفين على المادة: | 0 (Coloring Agents) |
| تواريخ الأحداث: | Date Created: 19970101 Date Completed: 19970527 Latest Revision: 20191101 |
| رمز التحديث: | 20221213 |
| DOI: | 10.3109/10520299709082206 |
| PMID: | 9062705 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1052-0295 |
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| DOI: | 10.3109/10520299709082206 |