دورية أكاديمية
Chloral hydrate formation in the Japanese medaka minnow.
| العنوان: | Chloral hydrate formation in the Japanese medaka minnow. |
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| المؤلفون: | Lipscomb JC; United States Air Force, Armstrong Laboratory, Toxicology Division, Wright-Patterson Air Force Base, OH 45433-7400, USA., Buttler GW, Confer PD |
| المصدر: | Annals of clinical and laboratory science [Ann Clin Lab Sci] 1997 Mar-Apr; Vol. 27 (2), pp. 157-62. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Institute for Clinical Science Country of Publication: United States NLM ID: 0410247 Publication Model: Print Cited Medium: Print ISSN: 0091-7370 (Print) Linking ISSN: 00917370 NLM ISO Abbreviation: Ann Clin Lab Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Philadelphia, Institute for Clinical Science. |
| مواضيع طبية MeSH: | Chloral Hydrate/*metabolism , Oryzias/*metabolism, Animals ; Cytochrome P-450 CYP2E1/metabolism ; Cytochrome P-450 Enzyme System/metabolism ; Microsomes, Liver/enzymology ; NADP/metabolism ; Trichloroethylene/metabolism ; Water Pollutants |
| مستخلص: | Trichloroethylene (TRI) is a common groundwater contaminant that has been shown to be tumorigenic and toxic in laboratory animals. The toxicity of TRI is increased by inducing the production of cytochrome P-450-dependent metabolites. Cytochrome P450 (CYP) 2E1 metabolizes TRI in mammals; however, this isoform of CYP2E1 does not appear to be expressed in fish. Medaka microsomal protein containing CYP was exposed to TRI and extracted with ethyl acetate. The extract was analyzed using gas chromatography (liquid injection) with an electron capture detector and separately using mass spectrometry. The formation of chloral hydrate, a precursor of toxic metabolites, was confirmed following exposure of hepatic microsomes of the medaka to TRI. These results indicate that medaka catalyze the first step in the formation of toxic metabolites and CYP forms in addition to CYP2E1 which catalyzes this reaction in fish. |
| المشرفين على المادة: | 0 (Water Pollutants) 290YE8AR51 (Trichloroethylene) 418M5916WG (Chloral Hydrate) 53-59-8 (NADP) 9035-51-2 (Cytochrome P-450 Enzyme System) EC 1.14.13.- (Cytochrome P-450 CYP2E1) |
| تواريخ الأحداث: | Date Created: 19970301 Date Completed: 19970612 Latest Revision: 20141120 |
| رمز التحديث: | 20231215 |
| PMID: | 9098516 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0091-7370 |
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