دورية أكاديمية
In vivo mitochondrial DNA-protein interactions in sea urchin eggs and embryos.
| العنوان: | In vivo mitochondrial DNA-protein interactions in sea urchin eggs and embryos. |
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| المؤلفون: | Roberti M; Dipartimento di Biochimica e Biologia Molecolare, Università di Bari and Centro Studi sui Mitocondri e Metabolismo Energetico, CNR, via Orabona 4A, I-70125, Bari, Italy., Polosa PL, Musicco C, Milella F, Qureshi SA, Gadaleta MN, Jacobs HT, Cantatore P |
| المصدر: | Current genetics [Curr Genet] 1999 Jan; Vol. 34 (6), pp. 449-58. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Country of Publication: United States NLM ID: 8004904 Publication Model: Print Cited Medium: Print ISSN: 0172-8083 (Print) Linking ISSN: 01728083 NLM ISO Abbreviation: Curr Genet Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York Ny : Springer International Original Publication: [New York] Springer International. |
| مواضيع طبية MeSH: | DNA, Mitochondrial/*genetics , DNA, Mitochondrial/*metabolism , DNA-Binding Proteins/*metabolism , Sea Urchins/*genetics , Sea Urchins/*metabolism, Animals ; Base Sequence ; Binding Sites/genetics ; Consensus Sequence ; DNA Footprinting ; Female ; Models, Genetic ; Molecular Sequence Data ; Ovum/metabolism ; RNA, Ribosomal/genetics ; RNA, Ribosomal, 16S/genetics ; Sea Urchins/embryology ; Sulfuric Acid Esters |
| مستخلص: | Footprinting studies with the purine-modifying agent dimethyl sulphate were performed in Paracentrotus lividus eggs and embryos to analyze in vivo the interactions between protein and mitochondrial DNA. Footprinting in the small non-coding region and at the boundary between the ND5 and ND6 genes revealed two strong contact sites corresponding with the in vitro binding sequences of mitochondrial D-loop-Binding Protein (mtDBP). The analysis of the pause region of mtDNA replication showed a strong footprint corresponding with the binding site of the mitochondrial Pause region-Binding Protein-2 (mtPBP-2), but only a very weak signal at the binding site of the mitochondrial Pause region-Binding Protein-1 (mtPBP-1), which in vitro binds DNA with high efficiency. In vitro and in vivo analysis of the 3' end-region of the two rRNA genes showed no significant protein-DNA interactions, suggesting that, in contrast to mammals, the 3' ends of sea urchin mitochondrial rRNAs are not generated by a protein-dependent transcription termination event. These and other data support a model in which expression of mitochondrial genes in sea urchins is regulated post-transcriptionally. Footprinting at the five AT-rich consensus regions allowed the detection of a binding site in the non-coding region for an as-yet unidentified protein, mtAT-1BP. The occupancy of this site appears to be developmentally regulated, being detectable in the pluteus larval stage, but not in unfertilized eggs. |
| معلومات مُعتمدة: | 863 Italy TI_ Telethon |
| المشرفين على المادة: | 0 (DNA, Mitochondrial) 0 (DNA-Binding Proteins) 0 (RNA, Ribosomal) 0 (RNA, Ribosomal, 16S) 0 (RNA, ribosomal, 12S) 0 (Sulfuric Acid Esters) JW5CW40Z50 (dimethyl sulfate) |
| تواريخ الأحداث: | Date Created: 19990205 Date Completed: 19990315 Latest Revision: 20220129 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/s002940050419 |
| PMID: | 9933356 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 0172-8083 |
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| DOI: | 10.1007/s002940050419 |