دورية أكاديمية
Inhibition of MMP-1 expression by antisense RNA decreases invasiveness of human chondrosarcoma
| العنوان: | Inhibition of MMP-1 expression by antisense RNA decreases invasiveness of human chondrosarcoma |
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| المؤلفون: | Jiang, Xiaoling, Dutton, Charyl M., Qi, Wen-ning, Block, Joel A., Brodt, Pnina, Durko, Margaret, Scully, Sean P. |
| المصدر: | Journal of Orthopaedic Research; Nov2003, Vol. 21 Issue 6, p1063, 8p |
| مصطلحات موضوعية: | ANTISENSE RNA, METALLOPROTEINASES, GENE expression |
| مستخلص: | We previously reported that an elevated level of matrix metalloproteinase-1 (MMP-1) gene expression in patients with chondrosarcoma has a strong statistical correlation with recurrence and in vitro invasion. In the present study, we used an antisense RNA strategy for MMP-1 inhibition to determine if this would affect the invasive characteristics of the cells. We transfected a human chondrosarcoma cell line with a retroviral plasmid expressing a 770 bp genomic fragment of the human MMP-1 gene in the sense or antisense orientation. The results show that cells transfected with the MMP-1 antisense fragment had a significant decrease in both MMP-1 protein and enzyme activity ( |
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| قاعدة البيانات: | Complementary Index |
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