دورية أكاديمية
Comparison of Diverse Differential Plating Methods to Enrich Bovine Spermatogonial Cells.
العنوان: | Comparison of Diverse Differential Plating Methods to Enrich Bovine Spermatogonial Cells. |
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المؤلفون: | Giassetti, MI, Goissis, MD, Barros, FRO, Bruno, AH, Assumpção, MEOA, Visintin, JA |
المصدر: | Reproduction in Domestic Animals; Feb2016, Vol. 51 Issue 1, p26-32, 7p |
مصطلحات موضوعية: | DOMESTIC animal reproduction, STEM cells, CALVES, GENETIC markers, SERUM albumin, FLOW cytometry |
مستخلص: | Contents Spermatogonial stem cells ( SSC) have important applications in domestic animal reproduction and advanced biotechnologies. Because differential plating is one of the most common methods used for SSC enrichment, the goal of this study was to compare three differential plating methods for the enrichment of bovine SSC. To achieve this goal, testicular parenchyma from pre-pubertal calves was minced and single cells were obtained after two enzymatic digestions. We compared three coating methods for differential plating: laminin (20 ng/ml), BSA (0.05 mg/ml) and PBS. Cells were incubated at 37°C, 5% CO |
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قاعدة البيانات: | Complementary Index |
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