التفاصيل البيبلوغرافية
| العنوان: |
Rapid Propagation of Chirita ophiopogoides in Vitro. |
| Alternate Title: |
条叶唇柱苣苔离体快繁技术研究. (Chinese) |
| المؤلفون: |
Chuanming FU, Kanghua XIAN, Jinxiang HE, Fengluan TANG, Yunping SHI, Ningzhen HUANG |
| المصدر: |
Agricultural Science & Technology; 2015, Vol. 16 Issue 12, p2677-2681, 5p |
| مصطلحات موضوعية: |
PLANT micropropagation, BUD development, SEEDLINGS, COMPOSITION of leaves, BANANAS, POTATOES, ROOTING of plant cuttings |
| Abstract (English): |
A method for in vitro culture and rapid propagation of Chirita ophiopogoides was developed using leaves as explants in this study. The results indicated that the medium MS+6-BA 0.1 mg/L+NAA 0.1 mg/L was suitable for bud induc- tion and seedling regeneration from leaves in primary culture. The media MS+0.5 mg/L 6-BA+0.1 mg/L NAA+10% banana+5% potato and MS+0.5 mg/L 6-BA+0.5 mg/L NAA+2% banana were very suitable for callus multiplication and seedling hardening in subculture, and the proliferation coefficients were 7.9 and 5.6 per 60 d respectively. The optimal rooting medium was MS and the rooting rate was 100% on day 30 of culture. The rooted plantlets of C. ophiopogoides were transplanted in greenhouse with humus soil and 92.5% survived. Theoretically, using the rapid propagation system, about 20 176 seedlings can be reproduced from a sterile plantlet in a year. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
以叶片为外植体,对条叶唇柱苣苔(Chirita ophiopogoides)进行离体培养与快速繁 殖研究。结果表明，培养基MS+0.1 mg/L 6- BA+0.1 mg/L NAA适用于初代培养中的芽诱 导和植株再生;培养基MS+0.5 mg/L 6-BA+0.1 mg/L NAA + 10%香蕉+5%马铃薯和 MS+0.5 mg/L 6-BA+0.5 mg/L NAA+2%香蕉分别适用 于继代增殖和壮苗培养，繁殖系数分别为7.9 倍/60 d和5.6倍/60 d;培养基MS适用于生 根培养，培养30 d后生根率达到100%;以腐 质土为基质，将条叶唇柱苣苔生根试管苗移栽 到大棚，成活率达92.5%。根据上述快繁技术， 理论上每株试管苗每年可繁殖条叶唇柱苣苔 种苗20 176株。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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