دورية أكاديمية
Mdivi-1 Inhibits Astrocyte Activation and Astroglial Scar Formation and Enhances Axonal Regeneration after Spinal Cord Injury in Rats.
| العنوان: | Mdivi-1 Inhibits Astrocyte Activation and Astroglial Scar Formation and Enhances Axonal Regeneration after Spinal Cord Injury in Rats. |
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| المؤلفون: | Gang Li, Yang Cao, Feifei Shen, Yangsong Wang, Liangjie Bai, Weidong Guo, Yunlong Bi, Gang Lv, Zhongkai Fan, Xiaojing J. Gao, Yu-Feng Wang |
| المصدر: | Frontiers in Cellular Neuroscience; 10/19/2016, Vol. 10, p1-14, 14p |
| مصطلحات موضوعية: | ASTROCYTES, SCARS, SPINAL cord injuries, AXONS, LABORATORY rats, MITOCHONDRIAL DNA, GLIAL fibrillary acidic protein, CHONDROITIN |
| مستخلص: | After spinal cord injury (SCI), astrocytes become hypertrophic, and proliferative, forming a dense network of astroglial processes at the site of the lesion. This constitutes a physical and biochemical barrier to axonal regeneration. Mitochondrial fission regulates cell cycle progression; inhibiting the cell cycle of astrocytes can reduce expression levels of axon growth-inhibitory molecules as well as astroglial scar formation after SCI. We therefore investigated how an inhibitor of mitochondrial fission, Mdivi-1, would affect astrocyte proliferation, astroglial scar formation, and axonal regeneration following SCI in rats. We stern blot and immunofluorescent double-labeling showed that Mdivi-1 markedly reduced the expression of the astrocyte marker glial fibrillary acidic protein (GFAP), and a cell proliferation marker, proliferating cell nuclear antigen, in astrocytes 3 days after SCI. Moreover, Mdivi-1 decreased the expression of GFAP and neurocan, a chondroitin sulfate proteoglycan. Notably, immunofluorescent labeling and Nissl staining showed that Mdivi-1 elevated the production of growth-associated protein-43 and increased neuronal survival at 4 weeks after SCI. Finally, hematoxylin-eosin staining, and behavioral evaluation of motor function indicated that Mdivi-1 also reduced cavity formation and improved motor function 4 weeks after SCI. Our results confirm that Mdivi-1 promotes motor function after SCI, and indicate that inhibiting mitochondrial fission using Mdivi-1 can inhibit astrocyte activation and astroglial scar formation and contribute to axonal regeneration after SCI in rats. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: | Complementary Index |
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