التفاصيل البيبلوغرافية
| العنوان: |
Membrane lipid peroxidation and redox status in erythrocytes of sickle cell anaemia patients incubated in leaf extract of Gongronema Latifolium. |
| المؤلفون: |
Chikezie, Paul Chidoka |
| المصدر: |
Journal of Experimental & Integrative Medicine; 2016, Vol. 6 Issue 2, p57-65, 9p |
| مصطلحات موضوعية: |
SICKLE cell anemia, LIPID peroxidation (Biology), ERYTHROCYTES, MILKWEEDS, PLANT extracts, MALONDIALDEHYDE, METHEMOGLOBIN, SPECTROPHOTOMETRY |
| مستخلص: |
Aim/Background: The present study sought to ascertain the capacity of ethanolic leaf extract of Gongronema latifolium to deter membrane lipid peroxidation and exert appropriate redox status in sickle erythrocytes using in vitro models. Methods: Erythrocyte malondialdehyde (MDA) concentration, membrane thiobarbituric acid reactive substances (TBARS) concentration, ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) (GSH/GSSG ratio), methaemoglobin (MetHb) concentration and NADH-methaemoglobin reductase (NADH-MR) activity were monitored in the presence of ethanolic leaf extract of G. latifolium for 15 h using spectrophotometric methods. Results: Erythrocyte MDA concentration of the control sample incubated for 15 h was within the range of 2.43 ± 0.19 - 3.54 ± 0.31 mmol/mL representing 45.67% increase in MDA concentration. The capacity of the extract to lower erythrocyte MDA concentration was in a concentration dependent manner. Erythrocytes incubated for 5 h in 100 mg/dL extract caused 8.59% reduction in erythrocyte membrane TBARS, whereas erythrocytes incubated for 5 h in 400 mg/dL extract gave 22.44%; p < 0.05. Within the experimental time intervals of: 5 h = t = 15 h, erythrocyte GSH/GSSG ratios of the test samples gave greater numerical values than that of the control sample. Notably, NADH-MR activity of erythrocytes incubated in 400 mg/dL extract increased within a relatively narrow range: 9.96 ± 1.22 - 10.05 ± 2.04 IU/gHb; p > 0.05. Conclusion: The present study showed that ethanolic leaf extract of G. latifolium deterred lipid peroxidation but did not completely obliterate the propensity of the sickle erythrocytes to undergo oxidative deterioration in vitro. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
