التفاصيل البيبلوغرافية
| العنوان: |
Free-radical Scavenging properties and Cytotoxic Activity Evaluation of Latex C-serum from Hevea brasiliensis RRIM 600. |
| المؤلفون: |
Kerche-Silva, Leandra Ernst, Cavalcante, Dalita Gomes Silva Morais, Danna, Caroline Silva, Gomes, Andressa Silva, Carrara, Iriana Moratto, Cecchini, Alessandra Lourenço, Yoshihara, Eidi, Job, Aldo Eloizo |
| المصدر: |
Free Radicals & Antioxidants; Jan-Jun2017, Vol. 7 Issue 1, p107-114, 8p |
| مصطلحات موضوعية: |
FREE radicals, RADICALS (Chemistry), CELL-mediated cytotoxicity, HEVEA, FOURIER transform infrared spectroscopy |
| مستخلص: |
Background: There are few studies exploring the antioxidant properties of the latex C-serum of the Hevea brasiliensis latex and their possible use for therapeutic application. Objective: This study investigated the in vitro antioxidant activity of the latex C-serum of H. brasiliensis and assessed the cytotoxicity of this fraction in two human cell lines derived from normal tissue (MRC-5 and CCD 1059sk. Chemical characterization was also assessed. Methods: Latex from H. brasiliensis was centrifuged and the C-serum phase was tested for in vitro free radical scavenging assays, such as hydroxyl and nitric oxide radicals, hydrogen peroxide and total antioxidant capacity by the phosphomolibdate method. Latex C-serum was also investigated for cytotoxic properties by the MTT test. Latex C-serum was chemical characterized by Infrared Spectroscopy Fourier Transform (FTIR). Results: Latex C-serum effectively scavenged hydroxyl and nitric oxide radicals and hydrogen peroxide and presented potent antioxidant activity. The results were compared to ascorbic acid, a standard antioxidant. The cytotoxicity evaluation of latex C-serum demonstrated cell line dependency and for CCD 1059sk cells derived from skin fibroblasts, latex C-serum was not cytotoxic. FTIR showed the presence of proteins in latex C-serum. Conclusion: Latex C-serum from Hevea brasiliensis presented strong antioxidant potential and these results openthe possibility of further studies aiming the use of latex C-serum as treatment for skin diseases related to reactive oxygenspecies or skin preventive pharmacological formulations. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
