دورية أكاديمية
OPTIMISATION OF POLYMERASE CHAIN REACTION CONDITIONS TO AMPLIFY D-LOOP REGION IN THE MALAYSIAN MOUSEDEER GENOMIC DNA.
| العنوان: | OPTIMISATION OF POLYMERASE CHAIN REACTION CONDITIONS TO AMPLIFY D-LOOP REGION IN THE MALAYSIAN MOUSEDEER GENOMIC DNA. |
|---|---|
| المؤلفون: | BAKAR, MOHAMAD-AZAM AKMAL ABU, ROVIE-RYAN, JEFFRINE JAPNING, AMPENG, AHMAD, YAAKOP, SALMAH, NOR, SHUKOR MD, MD-ZAIN, BADRUL MUNIR |
| المصدر: | Malaysian Applied Biology; Dec2017, Vol. 46 Issue 4, p63-71, 9p |
| مصطلحات موضوعية: | POLYMERASE chain reaction, CHEVROTAINS, CHROMATOGRAMS |
| مستخلص: | Polymerase chain reaction (PCR) has been utilized in molecular studies to amplify DNA fragments even from small amounts of genetic material. A good PCR product is based on good amplification of DNA segments in condition optimisation. We conducted a molecular study to optimize the annealing temperature and concentration of primers to find the optimal condition of PCR for Malaysian mousedeer species. DNA samples from six Tragulus kanchil and Tragulus napu species of Malaysian mousedeer were extracted for PCR amplification. Several ranges of annealing temperatures and concentrations were tested using the increment and decrement method. Results indicated that the optimal annealing temperatures for T. kanchil and T. napu were 56.0°C and 47.2°C, respectively. For primer concentration, 10 to 20 µM is best to produce good PCR amplification. All six samples for the D-loop region had good DNA chromatograms and scores between 90% and 100% in BLAST. Based on the high scores, all mousedeer samples were considered to be highly purified DNA. [ABSTRACT FROM AUTHOR] |
| Copyright of Malaysian Applied Biology is the property of Malaysian Society of Applied Biology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| قاعدة البيانات: | Complementary Index |
كن أول من يترك تعليقا!