Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls.

التفاصيل البيبلوغرافية
العنوان:	Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls.
المؤلفون:	Ferreira, C. E. R., Haas, C. S., Goularte, K. L., Gasperin, B. G., Lucia, Jr, T., Rovani, M. T., Schneider, A., Cardoso, F. F.
المصدر:	Andrologia; Apr2018, Vol. 50 Issue 3, p1-1, 6p
مصطلحات موضوعية:	PARAOXONASE, SEMINAL proteins, SERUM, ACUTE phase proteins, SPERM motility
مستخلص:	Summary: The paraoxonases types 1, 2 and 3 (PON1, PON2 and PON3, respectively) are enzymes that degrade lipid peroxides, preventing oxidative damages relevant for male reproductive function. This study determined the expression of those three paraoxonases in reproductive tissues of bulls and evaluated correlations among the activity of PON1 in the serum and seminal plasma with breeding soundness parameters in bulls. The expression of PON1, PON2 and PON3 was characterised by RT‐PCR in samples of testicular parenchyma, vesicular glands and epididymis collected from three slaughtered bulls. All three paraoxonases were expressed in the testicular parenchyma, PON2 and PON3 were both expressed in the epididymis head and PON3 was also expressed in the epididymis tail. The PON1 activity was determined in samples of serum and seminal plasma from 110 bulls submitted to breeding soundness evaluation. There was a strong correlation (r = .90) between the activity of the PON1 in both serum and seminal plasma (p < .0001). The PON1 activity in the seminal plasma was positively correlated with ejaculate's colour, sperm mass activity (p = .04), motility, vigour and viability (all p < .01). Thus, PON1 may be a potential marker for sperm motility and viability in bulls. [ABSTRACT FROM AUTHOR]
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قاعدة البيانات:	Complementary Index

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