التفاصيل البيبلوغرافية
| العنوان: |
脂质体介导VEGF基因对成骨细胞增殖、合成骨钙素 以及细胞周期的相关研究. (Chinese) |
| Alternate Title: |
Effect of Liposome Mediated VEGF Gene on Osteoblast Proliferation, Osteocalcin Synthesis and Cell Cycle. (English) |
| المؤلفون: |
熊军, 刘韦, 曾凡, 陈剑飞, 周海燕 |
| المصدر: |
Progress in Modern Biomedicine; Aug2018, Vol. 18 Issue 16, p3042-3045, 4p |
| مصطلحات موضوعية: |
VASCULAR endothelial growth factors, OSTEOBLASTS, CELL proliferation, OSTEOCALCIN genetics, LIPOSOMES |
| Abstract (English): |
Objective: To investigate the effects of vascular endothelial growth factor (VEGF) gene mediated by liposome on osteoblast proliferation, osteocalcin synthesis and cell cycle. Methods: The recombinant plasmid pcDNA3-hVEGF with exogenous VEGF was introduced into cultured osteoblasts by liposome-mediated gene transfection method. The concentration changes of VEGF were detected by enzyme linked immunosorbent assay (ELISA) in the transfected cells to determine transfection efficiency. The osteoblast proliferation were measured by Cell counting; the cell cycle were analyzed by flow cytometry; the expression of osteocalcin were detected by ELISA. Results: Compared with the control group, the concentration of VEGF in transfected osteoblasts increased significantly, and the difference was statistically significant (P<0.05). The proliferation of osteoblasts transfected with recombinant genes was significantly enhanced (P<0.05), Cell cycle (G2/M+S)% increased significantly, and the difference was statistically significant (P< 0.05); the concentration of synthetic osteocalcin was significantly increased, and the difference was statistically significant (P<0.05). Conclusion: Liposome-mediated osteoblasts increase vascular endothelial growth factor level, promote osteoblast proliferation and increase osteoblast osteocalcin concentration to improve osteoblast function. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的：研究脂质体介导血管内皮生长因子（VEGF）基因对成骨细胞增殖、合成骨钙素以及细胞周期的影响。方法：通过脂质体 介导的基因转染方法，将携带外源性VEGF重组pcDNA3-hVEGF 质粒导入体外培养的成骨细胞，酶联免疫吸附测定法(ELISA) 检测转染后细胞中VEGF浓度变化，以判断转染效果；采用细胞计数法检测转染重组质粒的成骨细胞的增殖活性；流式细胞术检 测转染重组质粒的成骨细胞周期的变化；ELISA 检测转染重组质粒的成骨细胞骨钙素浓度变化。结果：与对照组相比，转染组成 骨细胞中VEGF 的浓度显著增加，与对照组间差异具有统计学意义（P<0.05）；转染重组质粒的成骨细胞的增殖能力较对照组显著 增强，差异具有统计学意义（P<0.05），与对照组相比，转染重组质粒的成骨细胞周期（G2/M+S）%明显增加，差异具有统计学意义 （P<0.05）；转染重组质粒的成骨细胞合成的骨钙素浓度较对照组显著升高，差异具有统计学意义（P<0.05）。结论：脂质体介导成骨 细胞增加血管内皮生长因子的水平，可促进成骨细胞增殖，增加成骨细胞骨钙素的浓度，从而提高成骨细胞的功能。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
