Purification and characterization of two Cl- -activated aminopeptidases hydrolysing basic termini from human skeletal muscle.

التفاصيل البيبلوغرافية
العنوان:	Purification and characterization of two Cl^- -activated aminopeptidases hydrolysing basic termini from human skeletal muscle.
المؤلفون:	Mantle, David, Lauffart, Brenda, McDermott, John R., Kidd, Alison M., Pennington, J. T.
المصدر:	European Journal of Biochemistry; 3/1/85, Vol. 147 Issue 2, p307-312, 6p
مصطلحات موضوعية:	CHLORINE, AMINOPEPTIDASES, HUMAN skeleton, MUSCLES, PEPTIDES, AMINO acids
مستخلص:	Two aminopeptidases (I and II), hydrolysing basic termini, were purified to homogeneity (as judged by polyacrylamide gel electrophoresis) from human. quadriceps muscle by anion-exchange chromatography and preparative electrophoresis. The electrophoretic migration rate of II was approximately 80% of that of I. Both enzymes had the following properties: optimum activity was at pH 6.5; addition of 0.15 M Cl^- or Br^- anions resulted in 20-fold or 10-fold increase inactivity respectively. There was little or no increase in activity on the addition of other anions, or divalent cations (0.5 - mM). Approximately 50% inhibition of activity was obtained in the presence of bestatin (0.1μM), p-hydroxymercuriphenylsuphonic acid (0.1μM) EDTA (10 mM) 1,10-phenanthroline (100 &muM), N-ethylmalimide (1mM) and Bu^I-Thr-Phe-Pro (0.5mM). The molecular mass was 72000 Da (gel filtration). Only the arginyl and lysyl 7-amino-4-methylcoumarin (Amc) derivatives wer appreciablyhydrolysed; approximate Km values for the reaction of I and II with these substrates (10-250 μM) were estimated as follows: Arg-Amc, Km^I =70μM, Km^II= 270 μM; Lys-Amc Km^II =400 μM. Both enzymes hydrolysed sipeptides with Arg or Lys as the NH2-terminal amino acid, however this was not an absolute requirement for dipeptide hydrolysis. The action of I and II on physiologically active oligopeptides was very restricted, with only bradykinin, proangiotensin and neurotensin being appreciably degraded. The breakdown of these peptides did not occur by classical aminopeptidase action (i.e. hydrolysis of the NH2-terminal residues), but via cleavage of internal peptide bonds. These results suggest that I and II may be isoenzymes of a CI^--requiring, thiol-type aminopeptidase, which hydrolyses basic termini. These enzymes may act primarily as dipeptidases, with a very restrieted mode of action in the degradation of naturally occurring oligopeptides. [ABSTRACT FROM AUTHOR]
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قاعدة البيانات:	Complementary Index

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