دورية أكاديمية
EVALUATION OF GENETIC VARIATION AMONG SOME SORGHUM GENOTYPES USING SRAP AND RAPD MARKERS.
| العنوان: | EVALUATION OF GENETIC VARIATION AMONG SOME SORGHUM GENOTYPES USING SRAP AND RAPD MARKERS. |
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| المؤلفون: | Awaly, Sara B. H., Ahmed, Dalia S. |
| المصدر: | Plant Archives (09725210); Apr2020, Vol. 20 Issue 1, p2793-2801, 9p |
| مصطلحات موضوعية: | SORGHUM genetics, RAPD technique, SORGHUM breeding, GENETIC polymorphisms, CLUSTER analysis (Statistics) |
| مستخلص: | The sorghum breeding programs require to select and use of diverse cultivars to create genetic diversification. Therefore, the genetic variability among six sorghum cultivars was estimated by using twenty one SRAP primers combination and fourteen RAPD primers. The total number of amplified bands was 161 and 169 of which 94 and 148 polymorphic bands which appearing 58.4% and 87.6% polymorphism for SRAP and RAPD markers, respectively. Furthermore, the values of PIC (polymorphism information content) were accounted for each primer, to estimate the discriminatory vigor of the molecular markers for analysis of linkage and polymorphism. The PIC values for SRAP markers ranged from 0.60 by Me6 + Em6 to 0.15 by Me2 + Em3 and Me2 + Em4 with an average of 0.35 and the values of PIC for RAPD markers ranged from 0.81 by the OPP-08 primer to 0.41 by the OPE-20 primer. Similarity coefficients among the cultivars were ranged from 78.9% among Maka-244 and Horas cultivars to 61.3% among the Special-90 and Maka-244. The similarity coefficient was utilized to create a UPGMA based on pooled data from SRAP and RAPD analyses to illustrate the genetic distances among the six sorghum cultivars. These analyses demonstrated the power of SRAP and RAPD markers for estimating genetic diversification among sorghum cultivars. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: | Complementary Index |
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