DETECTION OF GENE BADH2 IN CHARGE OF AROMATICS IN IRAQI RICE VARIETIES.

التفاصيل البيبلوغرافية
العنوان:	DETECTION OF GENE BADH2 IN CHARGE OF AROMATICS IN IRAQI RICE VARIETIES.
المؤلفون:	Mohammed, Layla Ismail, Farhood, Ali Nadhim
المصدر:	Plant Archives (09725210); Oct2020, Vol. 20 Issue 2, p1077-1084, 8p
مصطلحات موضوعية:	GENE expression, POLYMERASE chain reaction, AROMATIC compounds, RICE varieties, FERTILIZERS, BIOSYNTHESIS
مستخلص:	Two field experiments were carried out during two agricultural seasons 2016 and 2017 in the rice research station in Mashkhab (province of Najaf Al-Ashraf) related to the Agricultural Research office. The agricultural season of 2016 included the cultivation of three varieties of rice, which they were Anber -33, jasmine, and Mashkhab -2, for the purpose of selection basis on aromatic for plants characterized by high aromatic of the two aromatic varieties (Anber 33 and Jasmine), and the detection of the badh2 gene in charge of aromatics in the three varieties using Polymerase Chain Reaction (PCR) technology and analysis of nucleotide sequences. While in the agricultural season 2017, and for the purpose of evaluating the performance of genotypes (selected and origin) by gene expression of badh-2 gene, and to know the role of chemical fertilization NPK by gene expression of badh-2 gene in Anbar 33 using Real time-quantitative polymerase chain reaction (RT-qPCR) technique. Randomized Complete Block Design (RCBD was used with three replicates, included 8 treatments was (Mashkhab 2 under the fertilization level NPK0, Jasmine origin under the fertilization level NPK0, Anber 33 origin under the fertilization level NPK0, Jasmine selected based on aromatic under the fertilization level NPK0, Anber 33 selected based on aromatic under the fertilization level NPK0, Anber 33 origin under fertilization level NPK1(70, 23, 25 kg N, P2O5, K2O h^-1), Anber 33 origin under fertilization level NPK2(140, 46, 50 kg N, P2O5 and K2O h^-1), Anber 33 origin under fertilization level NPK3(280, 92, 100 kg N, P2O5 and K2O h^-1). The results of PCR reaction showed that there are bands with a molecular weight of 752bp in the three varieties representing the badh-2 gene, the nitrogenous base sequence identical revealed that the badh2 gene in two aromatic varieties Anber 33 and Jasmine was truncated and incomplete, while it was completely in the non - aromatic variety. The results of RT-qPCR showed that the gene expression of badh2 gene decreased in the two aromatic varieties compared to the non - aromatic variety, in addition, the chemical fertilization (NPK2 and NPK3) caused a significant increase in the gene expression of badh2 in Anber 33. Therefore can conclude that the presence of allele badh2 dominant biosynthesis of 2acetylpyrrolne (2AP) which is the main aromatic compound causing aromatic for rice, whereas the truncated recessive allelees of the badh2 stimulate the formation of 2AP, and the expression of the badh-2 gene increases with increasing levels of chemical fertilization therefore decreases the aromatic degree. [ABSTRACT FROM AUTHOR]
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قاعدة البيانات:	Complementary Index

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