التفاصيل البيبلوغرافية
| العنوان: |
NON VIRULENT Escherichia coli O157:H43 IN NATIVE PIGS (Sus scrofa domestica Erxleben) FROM SELECTED FARMS IN CAVITE, PHILIPPINES. |
| المؤلفون: |
Rundina-Dela Cruz, Ma. Cynthia N., Dalmacio, Ida F., De Castro, Renzi Owell V. |
| المصدر: |
Philippine Journal of Veterinary Medicine; Jul-Dec2020, Vol. 57 Issue 2, p137-146, 10p |
| مصطلحات موضوعية: |
ESCHERICHIA coli, SWINE breeding, DOMESTIC animals, SWINE, GENES, GENE clusters |
| مستخلص: |
The study confirmed the presence of E. coli O157:H43 in the feces of native pigs from selected farms in General Trias, Cavite, Philippines. Twenty seven (27) presumptive EHEC organisms manifesting as colorless colonies with a smoky center on Cefixime Tellurite-Sorbitol MacConkey (CT-SMAC) Agar were subjected to morphological and biochemical characterization. The isolates were Gram negative rod-shaped organisms and possessed biochemical characteristics typical of E. coli except for three isolates that did not ferment the sugar sorbitol. Two of the three isolates agglutinated the O157 typing serum but not the H7 typing serum and this was confirmed through PCR. Moreover, PCR showed that none of the isolates harbored the virulence genes for Shiga toxin 1 (stx1), Shiga toxin 2 (stx2), intimin (eaeA), and hemolysin (hlyA). The isolates were also non-hemolytic. The BLAST search confirmed 100% identity with E. coli O157:H43 DNA, O antigen biosynthesis gene cluster, strain PV00-24 which represent a distinct class of E. coli O157 that are not pathogenic. A prevalence of 3.3% was obtained. To the best of the authors' knowledge, this is the first report of E. coli O157:H43 in native pigs and an E. coli O157 strain that is non-pathogenic in domestic animals in the country. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
