A Clinical Trial to Evaluate the Efficacy of α-Viniferin in Staphylococcus aureus - Specific Decolonization without Depleting the Normal Microbiota of Nares.

التفاصيل البيبلوغرافية
العنوان:	A Clinical Trial to Evaluate the Efficacy of α-Viniferin in Staphylococcus aureus - Specific Decolonization without Depleting the Normal Microbiota of Nares.
المؤلفون:	RAHIM, MD ABDUR, HOONHEE SEO, SUKYUNG KIM, YOON KYOUNG JEONG, TAJDOZIAN, HANIEH, MIJUNG KIM, SAEBIM LEE, HO-YEON SONG
المصدر:	Polish Journal of Microbiology; 2021, Vol. 70 Issue 1, p117-130, 14p
مصطلحات موضوعية:	METHICILLIN-resistant staphylococcus aureus, DECOLONIZATION, COMMUNITY-acquired infections, ANTIBACTERIAL agents, RNA sequencing, CLINICAL trials
مستخلص:	Staphylococcus aureus is currently a significant multidrug-resistant bacterium, causing severe healthcare-associated and communityacquired infections worldwide. The current antibiotic regimen against this pathogen is becoming ineffective due to resistance, in addition, they disrupt the normal microbiota. It highlights the urgent need for a pathogen-specific drug with high antibacterial efficacy against S. aureus. α-Viniferin, a bioactive phytochemical compound, has been reported to have excellent anti-Staphylococcus efficacy as a topical agent. However, so far, there were no clinical trials that have been conducted to elucidate its efficacy. The present study aimed to investigate the antibacterial efficacy of α-viniferin against S. aureus in a ten-day clinical trial. Based on the results, α-viniferin showed 50% minimum inhibitory concentrations (MIC50 values) of 7.8 μg/ml in culture broth medium. α-Viniferin was administered in the nares three times a day for ten days using a sterile cotton swab stick. Nasal swab specimens were collected before (0 days) and after finishing the trial (10^th day), and then analyzed. In the culture and RT-PCR-based analysis, S. ureus was reduced significantly: 0.01. In addition, 16S ribosomal RNAbased amplicon sequencing analysis showed that S. aureus reduced from 51.03% to 23.99% at the genus level. RNA-seq analysis was also done to gain insights into molecular mechanisms of α-viniferin against S. aureus, which revealed that some gene groups were reduced in 5-fold FC cutoff at two times MIC conditions. The study results demonstrate α-viniferin as a potential S. aureus-specific drug candidate. [ABSTRACT FROM AUTHOR]
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قاعدة البيانات:	Complementary Index

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