التفاصيل البيبلوغرافية
| العنوان: |
核磁共振磷谱法测定乳制品中的酪蛋白含量. (Chinese) |
| Alternate Title: |
Determination of Casein Content in Dairy Products by 31P Nuclear Magnetic Resonance. (English) |
| المؤلفون: |
冯翠萍, 刘一诺, 樊双喜, 武竹英, 钟其顶 |
| المصدر: |
Shipin Kexue/ Food Science; 2021, Vol. 42 Issue 4, p221-226, 6p |
| مصطلحات موضوعية: |
NUCLEAR magnetic resonance, DAIRY product marketing, DAIRY products, REQUIREMENTS engineering, STANDARD deviations, CASEINS |
| Abstract (English): |
In this study, we established a quantitative method for the detection of casein in dairy products using 31P nuclear magnetic resonance (NMR) combined with the interpolation method. The results showed that the limit of detection (LOD) of this method was 0.38 g/L (RSN = 3), and the limit of quantitation (LOQ) was 1.25 g/L (RSN = 10). Good linearity was observed within the concentration range of 5.00–35.00 g/L, with correlation coefficient R² greater than 0.999. The spiked recoveries were in the range of 91.94%–105.10%. The intra-day and inter-day precisions (relative standard deviation, RSD) were in the range of 0.65%–1.40% and 1.40%–1.80%, respectively. The casein contents of different dairy products on the market were detected by this method and the current national standard method (GB 31638‒2016). The error between the two methods was within ± 5%, which met the requirements for comparative analysis and validation of feasibility. Compared with the conventional method, this method had the advantages of simpler sample pretreatment, higher quantitative accuracy, greatly shorter detection time and wider applicability. Therefore, it could be suitable for rapid determination of casein content in dairy products. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
基于插值法建立乳制品中酪蛋白的核磁共振磷谱定量检测方法。结果表明，该方法的检出限为 0.38 g/L （信噪比（RSN）=3），定量限为 1.25 g/L（RSN=10）；在 5.00～35.00 g/L质量浓度范围内线性良好，相关系数 R² 大于0.999；加标回收率在 91.94%～105.10% 范围区间；日内精密度在 0.65%～1.40%范围区间；日间精密度在 1.40%～1.80% 范围区间。对市售不同乳制品中酪蛋白含量进行检测，该方法与 GB 31638—2016《酪蛋白》测定结 果误差在 ±5%以内，满足方法可行性对比分析验证要求。该方法相比常规方法样品前处理简单、定量准确性高， 大大缩短了检测时间，且有更广泛的适用性，满足乳制品中酪蛋白快速定量检测的要求。 [ABSTRACT FROM AUTHOR] |
|
Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
