التفاصيل البيبلوغرافية
| العنوان: |
Dynamic intron retention modulates gene expression in the monocytic differentiation pathway. |
| المؤلفون: |
Song, Renhua, Tikoo, Shweta, Jain, Rohit, Pinello, Natalia, Au, Amy Y. M., Nagarajah, Rajini, Porse, Bo, Rasko, John E. J., J.‐L. Wong, Justin |
| المصدر: |
Immunology; Feb2022, Vol. 165 Issue 2, p274-286, 13p |
| مصطلحات موضوعية: |
GENE expression, BONE marrow, MONOCYTES, IMMUNE response |
| مستخلص: |
Monocytes play a crucial role in maintaining homeostasis and mediating a successful innate immune response. They also act as central players in diverse pathological conditions, thus making them an attractive therapeutic target. Within the bone marrow, monocytes arise from a committed precursor termed Common Monocyte Progenitor (cMoP). However, molecular mechanisms that regulate the differentiation of cMoP to various monocytic subsets remain unclear. Herein, we purified murine myeloid precursors for deep poly‐A‐enriched RNA sequencing to understand the role of alternative splicing in the development and differentiation of monocytes under homeostasis. Our analyses revealed intron retention to be the major alternative splicing mechanism involved in the monocyte differentiation cascade, especially in the differentiation of Ly6Chi monocytes to Ly6Clo monocytes. Furthermore, we found that the intron retention of key genes involved in the differentiation of murine Ly6Chi to Ly6Clo monocytes was also conserved in humans. Our data highlight the unique role of intron retention in the regulation of the monocytic differentiation pathway. [ABSTRACT FROM AUTHOR] |
|
Copyright of Immunology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder