التفاصيل البيبلوغرافية
| العنوان: |
Abnormal hsa_circ_0003948 expression affects chronic periodontitis development by regulating miR‐144‐3p/NR2F2/PTEN signaling. |
| المؤلفون: |
Li, Wei, Zhang, Zhi, Li, Yizi, Wang, Zuomin |
| المصدر: |
Journal of Periodontal Research; Apr2022, Vol. 57 Issue 2, p316-323, 8p, 1 Chart, 5 Graphs |
| مصطلحات موضوعية: |
CHRONIC diseases, PERIODONTITIS, APOPTOSIS, GENE expression, CELLULAR signal transduction, BIOINFORMATICS, T-test (Statistics), CELL proliferation, BIOLOGICAL assay |
| مستخلص: |
Background and Objective: This study aimed to investigate the correlation between chronic periodontitis (CP) and abnormal circular RNA (circRNA) expression and to identify the role of hsa_circ_0003948 in the progression of CP. Methods: Next‐generation sequencing was utilized to investigate abnormal expression of circRNA in gingival tissues from CP patients and healthy control subjects. Bioinformatics and luciferase reporting analyses were used to clarify the interactive relationship among circRNA, miRNA, and mRNA. Periodontal ligament cells (PDLCs) were employed to analyze proliferation and apoptosis after lipopolysaccharide (LPS) treatment using the cell counting kit 8 (CCK8) assay and flow cytometry detection. Results: High‐throughput sequencing and RT‐qPCR analyses confirmed that hsa_circ_0003948 expression decreased dramatically in gingival samples of CP patients. Overexpression of hsa_circ_0003948 alleviated LPS‐induced PDLC injury by regulating NR2F2/PTEN signaling. The miR‐144‐3p and NR2F2 were determined to be hsa_circ_0003948 downstream targets. NR2F2 downregulation or miR‐144‐3p overexpression reversed the protective effect of hsa_circ_0003948 in PDLCs after treatment with LPS. Upregulation of NR2F2 reversed the inhibitory effect of miR‐144‐3p on surviving PDLCs after LPS treatment. Conclusion: Overexpression of hsa_circ_0003948 exerts a protective effect in CP via miR‐144‐3p/NR2F2/PTEN signaling regulation. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Periodontal Research is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder