التفاصيل البيبلوغرافية
| العنوان: |
Epithelial Na+ channels and stomatin are expressed in rat trigeminal mechanosensory neurons. |
| المؤلفون: |
Fricke, B., Lints, R., Stewart, G., Drummond, H., Dodt, G., Driscoll, M., von Düring, M. |
| المصدر: |
Cell & Tissue Research; Mar2000, Vol. 299 Issue 3, p327-334, 8p |
| مصطلحات موضوعية: |
SODIUM channels, SENSORY neurons, TRIGEMINAL nerve, ION channels, GASSERIAN ganglion, CAENORHABDITIS elegans, LABORATORY rats, IMMUNOCYTOCHEMISTRY |
| مستخلص: |
Caenorhabditis elegans MEC-4 and MEC-10 are subunits of the degenerin/epithelial Na+ channel (DEG/ENaC) ion channel superfamily thought to be associated with MEC-2 (a stomatin-like protein) in a mechanotransducing molecular complex in specialized touch sensory neurons. A key question is whether analogous molecular complexes in higher organisms transduce mechanical signals. To address this question, we selected mechanoreceptors of the rat vibrissal follicle-sinus complex in the mystacial pad and the trigeminal ganglia for an immunocytochemical and molecular biological study. RT-PCR of poly(A+) mRNA of rat trigeminal ganglia indicated that α-, β-, and γ-ENaC and stomatin mRNA are expressed in rat trigeminal ganglia. Using immunocytochemistry, we found that α-, β-, and γ-ENaC subunits and stomatin are localized in the perikarya of the trigeminal neurons and in a minor fraction of their termination site in the vibrissal follicle-sinus complex, where longitudinal lanceolate endings are immunopositive. We conclude that α-, β-, and γ-ENaC subunits as well as the candidate interacting protein stomatin are coexpressed in a mammalian mechanoreceptor, a location consistent with a possible role in mechanotransduction. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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