دورية أكاديمية
أعرض في EDS

Ry chc Confers Extreme Resistance to Potato virus Y in Potato.

التفاصيل البيبلوغرافية
العنوان: Ry chc Confers Extreme Resistance to Potato virus Y in Potato.
المؤلفون: Li, Gege, Shao, Jingjing, Wang, Yuwen, Liu, Tengfei, Tong, Yuhao, Jansky, Shelley, Xie, Conghua, Song, Botao, Cai, Xingkui
المصدر: Cells (2073-4409); Aug2022, Vol. 11 Issue 16, p2577, 19p
مصطلحات موضوعية: POTATO virus Y, SEED potatoes, PLANT viruses, POTATOES, POTATO industry, POTATO seeds
مستخلص: The Potato virus Y (PVY) is responsible for huge economic losses for the potato industry worldwide and is the fifth most consequential plant virus globally. The main strategies for virus control are to limit aphid vectors, produce virus-free seed potatoes, and breed virus-resistant varieties. The breeding of PVY-resistant varieties is the safest and most effective method in terms of cost and environmental protection. Rychc, a gene that confers extreme resistance to PVY, is from S. chacoense, which is a wild diploid potato species that is widely used in many PVY-resistant breeding projects. In this study, Rychc was fine mapped and successfully cloned from S. chacoense accession 40-3. We demonstrated that Rychc encodes a TIR-NLR protein by stably transforming a diploid susceptible cultivar named AC142 and a tetraploid potato variety named E3. The Rychc conferred extreme resistance to PVYO, PVYN:O and PVYNTN in both of the genotypes. To investigate the genetic events occurring during the evolution of the Rychc locus, we sequenced 160 Rychc homologs from 13 S. chacoense genotypes. Based on the pattern of sequence identities, 160 Rychc homologs were divided into 11 families. In Family 11 including Rychc, we found evidence for Type I evolutionary patterns with frequent sequence exchanges, obscured orthologous relationships and high non-synonymous to synonymous substitutions (Ka/Ks), which is consistent with rapid diversification and positive selection in response to rapid changes in the PVY genomes. Furthermore, a functional marker named MG64-17 was developed in this study that indicates the phenotype with 100% accuracy and, therefore, can be used for marker-assisted selection in breeding programs that use S. chacoense as a breeding resource. [ABSTRACT FROM AUTHOR]
Copyright of Cells (2073-4409) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
قاعدة البيانات: Complementary Index
كن أول من يترك تعليقا!
يجب تسجيل دخولك أولا