دورية أكاديمية
Characterization of Bacterial Community Structure Dynamics in a Rat Burn Wound Model Using 16S rRNA Gene Sequencing.
| العنوان: | Characterization of Bacterial Community Structure Dynamics in a Rat Burn Wound Model Using 16S rRNA Gene Sequencing. |
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| المؤلفون: | Zheng-li, Chen, Yu, Peng, Guo-sheng, Wu, Xu-Dong, Hong, Hao, Fan, Xu-dong, Zhang, Guang-Yi, Wang, Shi-hui, Zhu, Jian, Jin |
| المصدر: | Journal of Burn Care & Research; Sep/Oct2022, Vol. 43 Issue 5, p1086-1094, 9p |
| مصطلحات موضوعية: | TREATMENT for burns & scalds, WOUND infections, ANIMAL experimentation, RNA, RATS, GENES, RESEARCH funding, BACTERIAL diseases, BACTERIA |
| مستخلص: | Burns destroy the skin barrier and alter the resident bacterial community, thereby facilitating bacterial infection. To treat a wound infection, it is necessary to understand the changes in the wound bacterial community structure. However, traditional bacterial cultures allow the identification of only readily growing or purposely cultured bacterial species and lack the capacity to detect changes in the bacterial community. In this study, 16S rRNA gene sequencing was used to detect alterations in the bacterial community structure in deep partial-thickness burn wounds on the back of Sprague-Dawley rats. These results were then compared with those obtained from the bacterial culture. Bacterial samples were collected prior to wounding and 1, 7, 14, and 21 days after wounding. The 16S rRNA gene sequence analysis showed that the number of resident bacterial species decreased after the burn. Both resident bacterial richness and diversity, which were significantly reduced after the burn, recovered following wound healing. The dominant resident strains also changed, but the inhibition of bacterial community structure was in a nonvolatile equilibrium state, even in the early stage after healing. Furthermore, the correlation between wound and environmental bacteria increased with the occurrence of burns. Hence, the 16S rRNA gene sequence analysis reflected the bacterial condition of the wounds better than the bacterial culture. 16S rRNA sequencing in the Sprague-Dawley rat burn model can provide more information for the prevention and treatment of burn infections in clinical settings and promote further development in this field. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: | Complementary Index |
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