التفاصيل البيبلوغرافية
| العنوان: |
NAD+ metabolism drives astrocyte proinflammatory reprogramming in central nervous system autoimmunity. |
| المؤلفون: |
Meyer, Tom, Shimon, Dor, Youssef, Sawsan, Yankovitz, Gal, Tessler, Adi, Chernobylsky, Tom, Gaoni-Yogev, Anat, Perelroizen, Rita, Budick-Harmelin, Noga, Steinman, Lawrence, Mayo, Lior |
| المصدر: |
Proceedings of the National Academy of Sciences of the United States of America; 8/30/2022, Vol. 119 Issue 35, p1-12, 29p |
| مصطلحات موضوعية: |
CENTRAL nervous system, CENTRAL nervous system diseases, NEUROGLIA, JOB shops, AUTOIMMUNITY |
| مستخلص: |
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS). Astrocytes are the most abundant glial cells in the CNS, and their dysfunction contributes to the pathogenesis of MS and its animal model, experimental autoimmune encephalomyelitis (EAE). Recent advances highlight the pivotal role of cellular metabolism in programming immune responses. However, the underlying immunometabolic mechanisms that drive astrocyte pathogenicity remain elusive. Nicotinamide adenine dinucleotide (NAD+) is a vital coenzyme involved in cellular redox reactions and a substrate for NAD+-dependent enzymes. Cellular NAD+ levels are dynamically controlled by synthesis and degradation, and dysregulation of this balance has been associated with inflammation and disease. Here, we demonstrate that cell-autonomous generation of NAD+ via the salvage pathway regulates astrocyte immune function. Inhibition of nicotinamide phosphoribosyltransferase (NAMPT), a key enzyme in the salvage pathway, results in depletion of NAD+, inhibits oxidative phosphorylation, and limits astrocyte inflammatory potential. We identified CD38 as the main NADase up-regulated in reactive mouse and human astrocytes in models of neuroinflammation and MS. Genetic or pharmacological blockade of astrocyte CD38 activity augmented NAD+ levels, suppressed proinflammatory transcriptional reprogramming, impaired chemotactic potential to inflammatory monocytes, and ameliorated EAE. We found that CD38 activity is mediated via calcineurin/NFAT signaling in mouse and human reactive astrocytes. Thus, NAMPT-NAD+-CD38 circuitry in astrocytes controls their ability to meet their energy demands and drives the expression of proinflammatory transcriptional modules, contributing to CNS pathology in EAE and, potentially, MS. Our results identify candidate therapeutic targets in MS. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
