دورية أكاديمية
Citric Acid Induces the Increase in Lenthionine Content in Shiitake Mushroom, Lentinula edodes.
| العنوان: | Citric Acid Induces the Increase in Lenthionine Content in Shiitake Mushroom, Lentinula edodes. |
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| المؤلفون: | Hong, Mengting, Han, Dan, Qiao, Jinjin, Zhou, Xiaolin, Yu, Hanshou, Shi, Liang |
| المصدر: | Foods; Dec2022, Vol. 11 Issue 24, p4110, 14p |
| مصطلحات موضوعية: | CITRIC acid, SHIITAKE, EDIBLE fungi, MUSHROOMS, TREATMENT duration |
| مستخلص: | Shiitake mushroom, Lentinula edodes, is the second largest edible fungus in the world, with a characteristic aroma. 1,2,3,5,6-pentathioheterocycloheptane, commonly known as lenthionine, is the main source of this aroma. Lenthionine has high commercial value, and if we explore the possible induction mechanism of citric acid in lenthionine synthesis, we can provide a reference for the effective application of citric acid as an inducer. In this paper, the single-factor treatment of Lentinula edodes with variable citric acid concentration and treatment duration showed that the best citric acid concentration for L. edodes was 300 μM, and the best treatment duration was 15 days. Additionally, the optimal design conditions were obtained using the response surface method (RSM); the treatment concentration was 406 μM/L, the treatment duration was 15.6 days, and the lenthionine content was 130 μg/g. γ-Glutamyl transpeptidase (LEGGT) and cystine sulfoxide lyase (LECSL) are the key enzymes involved in the biosynthesis of lanthionine. The expression levels of LEGGT and LECSL genes increased significantly under citric acid treatment. Additionally, the lenthionine content of the silenced strains of LEGGT and LECSL was significantly decreased. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: | Complementary Index |
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