دورية أكاديمية
Diagnostic Accuracy of Methods for Detection of Antibodies against Type I Interferons in Patients with Endocrine Disorders.
| العنوان: | Diagnostic Accuracy of Methods for Detection of Antibodies against Type I Interferons in Patients with Endocrine Disorders. |
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| المؤلفون: | Nuralieva, Nurana, Yukina, Marina, Sozaeva, Leila, Donnikov, Maxim, Kovalenko, Liudmila, Troshina, Ekaterina, Orlova, Elizaveta, Gryadunov, Dmitry, Savvateeva, Elena, Dedov, Ivan |
| المصدر: | Journal of Personalized Medicine; Dec2022, Vol. 12 Issue 12, p1948, 10p |
| مصطلحات موضوعية: | TYPE I interferons, ENDOCRINE diseases, AUTOANTIBODIES, IMMUNOGLOBULINS, ENZYME-linked immunosorbent assay, ANTIBODY titer |
| مستخلص: | Autoantibodies against type 1 interferons (IFN-I) are a highly specific marker for type 1 autoimmune polyglandular syndrome (APS-1). Moreover, determination of antibodies to omega-interferon (IFN-ω) and alpha2-interferon (IFN-α2) allows a short-term diagnosis in patients with isolated and atypical forms of APS-1. In this study, a comparison of three different methods, namely multiplex microarray-based, cell-based and enzyme-linked immunosorbent assays for detection of antibodies against omega-interferon and alpha2-interferon, was carried out. A total of 206 serum samples from adult patients with APS-1, APS-2, isolated autoimmune endocrine pathologies or non-autoimmune endocrine disorders, and healthy individuals were analyzed. In the APS-1 patient cohort (n = 18), there was good agreement between the results of anti-IFN-I antibody tests performed by three methods, with 100% specificity and sensitivity for microarray-based assay. Although only the cell-based assay can determine the neutralizing activity of autoantibodies, the microarray-based assay can serve as a highly specific and sensitive screening test to identify anti-IFN-I antibody positive patients. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: | Complementary Index |
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