التفاصيل البيبلوغرافية
| العنوان: |
Great diversity of KSa sequences from bat-associated microbiota suggests novel sources of uncharacterized natural products. |
| المؤلفون: |
Salazar-Hamm, Paris S., Hathaway, Jennifer J. Marshall, Winter, Ara S., Caimi, Nicole A., Buecher, Debbie C., Valdez, Ernest W., Northup, Diana E. |
| المصدر: |
FEMS Microbes; 2022, Vol. 3, p1-11, 11p |
| مصطلحات موضوعية: |
NATURAL products, ACTINOBACTERIA, PUBLIC health, STREPTOMYCES, BATS |
| مستخلص: |
Polyketide synthases (PKSs) are multidomain enzymes in microorganisms that synthesize complex, bioactive molecules. PKS II systems are iterative, containing only a single representative of each domain: ketosynthase alpha (KSa), ketosynthase beta and the acyl carrier protein. Any gene encoding for one of these domains is representative of an entire PKS II biosynthetic gene cluster (BGC). Bat skin surfaces represent an extreme environment prolific in Actinobacteria that may constitute a source for bioactive molecule discovery. KSa sequences were obtained from culturable bacteria from bats in the southwestern United States. From 467 bat bacterial isolates, we detected 215 (46%) had KSa sequences. Sequencing yielded 210 operational taxonomic units, and phylogenetic placement found 45 (21%) shared <85% homology to characterized metabolites. Additionally, 16 Actinobacteria genomes from the bat microbiome were analyzed for biosynthetic capacity. A range of 69-93% of the BGCs were novel suggesting the batmicrobiomemay contain valuable uncharacterized natural products. Documenting and characterizing these are important in understanding the susceptibility of bats to emerging infectious diseases, such as white-nose syndrome. Also noteworthy was the relationship between KS a homology and total BGC novelty within each fully sequenced strain. We propose amplification and detection of KSa could predict a strain's global biosynthetic capacity. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
