دورية أكاديمية
K63‐linked polyubiquitination of LGP2 by Riplet regulates RIG‐I‐dependent innate immune response.
| العنوان: | K63‐linked polyubiquitination of LGP2 by Riplet regulates RIG‐I‐dependent innate immune response. |
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| المؤلفون: | Kouwaki, Takahisa, Nishimura, Tasuku, Wang, Guanming, Nakagawa, Reiko, Oshiumi, Hiroyuki |
| المصدر: | EMBO Reports; 2/6/2023, Vol. 24 Issue 2, p1-17, 17p |
| مستخلص: | Type I interferons (IFNs) exhibit strong antiviral activity and induce the expression of antiviral proteins. Since excessive expression of type I IFNs is harmful to the host, their expression should be turned off at the appropriate time. In this study, we find that post‐translational modification of LGP2, a member of the RIG‐I‐like receptor family, modulates antiviral innate immune responses. The LGP2 protein undergoes K63‐linked polyubiquitination in response to cytoplasmic double‐stranded RNAs or viral infection. Our mass spectrometry analysis reveals the K residues ubiquitinated by the Riplet ubiquitin ligase. LGP2 ubiquitination occurs with a delay compared to RIG‐I ubiquitination. Interestingly, ubiquitination‐defective LGP2 mutations increase the expression of type I IFN at a late phase, whereas the mutant proteins attenuate other antiviral proteins, such as SP100, PML, and ANKRD1. Our data indicate that delayed polyubiquitination of LGP2 fine‐tunes RIG‐I‐dependent antiviral innate immune responses at a late phase of viral infection. Synopsis: LGP2 undergoes K63‐linked ubiquitylation by Riplet at later stages of virus infection. Ubiquitylated LGP2 attenuates RIG‐I induced type I IFN expression and promotes antiviral protein expression. Riplet E3 ubiquitin ligase mediates K63‐linked polyubiquitination of LGP2.LGP2 ubiquitination occurs with a delay compared to RIG‐I ubiquitination during viral infection.Ubiquitinated LGP2 attenuates RIG‐I‐induced excessive type I IFN expression at a late phase of viral infection but promotes the expression of several antiviral proteins. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: | Complementary Index |
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