دورية أكاديمية
Delphinidin induces autophagic flux blockage and apoptosis by inhibiting both multidrug resistance gene 1 and DEAD-box helicase 17 expressions in liver cancer cells.
العنوان: | Delphinidin induces autophagic flux blockage and apoptosis by inhibiting both multidrug resistance gene 1 and DEAD-box helicase 17 expressions in liver cancer cells. |
---|---|
المؤلفون: | Sun, Shenghui, Xu, Kun, Yan, Mingjing, Cui, Ju, Zhu, Kaiyi, Yang, Yao, Zhang, Xiaoyi, Tang, Weiqing, Huang, Xiuqing, Dou, Lin, Chen, Beidong, Lin, Yajun, Zhang, Xiyue, Man, Yong, Li, Jian, Shen, Tao |
المصدر: | Journal of Pharmacy & Pharmacology; Feb2023, Vol. 75 Issue 2, p253-263, 11p |
مصطلحات موضوعية: | LIVER cells, GENE expression, MULTIDRUG resistance, LIVER cancer, CANCER cells |
مستخلص: | Objectives: To investigate the function and regulatory mechanisms of delphinidin in the treatment of hepatocellular carcinoma. Methods: HepG2 and HuH-7 cells were treated with different concentrations of delphinidin. Cell viability was analysed by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The cell autophagy and autophagic flux were analysed by LC3b-green fluorescent protein (GFP)-Adv and LC3b-GFP-monomeric red fluorescent protein-Adv transfected HepG2 and HuH-7 cells, respectively. Cell apoptosis was analysed by Hoechst33342 staining, terminal deoxynucleotidyl transferase dUTP nick end labeling staining and DNA laddering. Cell autophagy, apoptosis and survival related protein expressions were detected by Western blotting. Key findings: After treatment with different concentrations of delphinidin, the cell survival rate was significantly decreased. Delphinidin could block the autophagic flux, resulting in a significant increase in autophagosomes, and led to an increase in cell apoptosis. The combined application of delphinidin and cisplatin could promote the antitumour effect and reduce the dose of cisplatin in tumour cells. Further mechanism studies reveal that delphinidin could inhibit the multidrug resistance gene 1 (MDR1) and the tumour-promoting transcription cofactor DEAD-box helicase 17 (DDX17) expression in tumour cells. Overexpression of DDX17 could reverse delphinidin's antitumor function in tumour cells. Conclusions: Delphinidin has a strong anti-tumour effect by inducing tumour cell autophagic flux blockage and apoptosis by inhibiting of both MDR1 and DDX17 expression. [ABSTRACT FROM AUTHOR] |
Copyright of Journal of Pharmacy & Pharmacology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
قاعدة البيانات: | Complementary Index |
كن أول من يترك تعليقا!