التفاصيل البيبلوغرافية
| العنوان: |
Novel Nanobiocatalyst Constituted by Lipase from Burkholderia cepacia Immobilized on Graphene Oxide Derived from Grape Seed Biochar. |
| المؤلفون: |
de Almeida, Lays C., Andrade, Erikles L. O., Santos, Jefferson C. B., Santos, Roberta M., Fricks, Alini T., Freitas, Lisiane dos S., Lima, Álvaro S., Pereira, Matheus M., Soares, Cleide M. F. |
| المصدر: |
C; Mar2023, Vol. 9 Issue 1, p12, 13p |
| مصطلحات موضوعية: |
GRAPE seeds, GRAPHENE oxide, BURKHOLDERIA cepacia, LIPASES, BIOCHAR, GRAPES |
| مستخلص: |
The present research aims to study the process of immobilization of lipase from Burkholderia cepacia by physical adsorption on graphene oxide derived (GO) from grape seed biochar. Additionally, the modified Hummers method was used to obtain the graphene oxide. Moreover, Fourier transform infrared spectroscopy, Raman spectrum, X-ray diffraction, and point of zero charge were used for the characterization of the GO. The influences of pH, temperature, enzyme/support concentration on the catalytic activity were evaluated for the immobilized biocatalyst. The best immobilization was found (543 ± 5 U/g of support) in the pH 4.0. Considering the biochemical properties, the optimal pH and temperature were 3.0 and 50 °C, respectively, for the immobilized biocatalyst. Reusability studies exhibited that the immobilized lipase well kept 60% of its original activity after 5 cycles of reuse. Overall, these results showed the high potential of graphene oxide obtained from biochar in immobilization lipase, especially the application of nanobiocatalysts on an industrial scale. [ABSTRACT FROM AUTHOR] |
|
Copyright of C is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder