التفاصيل البيبلوغرافية
| العنوان: |
DOR 患者卵泡颗粒细胞/未成熟卵母细胞端粒 长度观察, 差异表达基因筛选及生物学功能分析. (German) |
| Alternate Title: |
Observation of telomere length in immature oocytes and granulosa cells of DOR patients, screening of differentially expressed genes in granulosa cells, and bioinformatics analysis. (English) |
| المؤلفون: |
陈海霞, 白晓红 |
| المصدر: |
Shandong Medical Journal; 4/25/2023, Vol. 63 Issue 12, p1-5, 5p |
| Abstract (English): |
Objective To observe the telomere length of immature oocytes (GV) and granulosa cells (GC) in diminished ovarian reserve (DOR) patients, to screen the differentially expressed genes in granulosa cells, and to analyze the biological functions of differentially expressioned genes. Methods We used quantitative polymerase chain reaction (qPCR) to detect the telomere length of GC and GV in 4 patients with DOR (DOR group) and 4 healthy controls with normal ovarian function (normal group). High-throughput sequencing was used to analyze the differentially expressed genes in GC of 4 cases of DOR group and 4 cases of normal group, and biological function was analyzed. Results The telomere length of GV in the DOR and normal groups was 0. 935 and 1. 39, respectively, while the telomere length of GC was 0. 915 and 1. 34, respectively, with statistically significant differences between the DOR group and normal group (all P<0. 05). Totally 997 up-regulated genes and 525 down-regulated genes were found in the DOR group in comparison with the normal group. GO analysis found that the significantly enriched differentially expressed genes involved in cell adhesion, cell division, negative regulation of BMP signaling pathway, positive regulation of apoptosis, negative regulation of cell proliferation, ATP binding, cell junction, mitochondrial matrix, catalytic activity, protease binding, mitochondria, inner mitochondrial membrane, oxidoreductase activity, response to cAMP, etc. KEGG items included cAMP signaling pathway, cGMP PKG signaling pathway, insulin secretion, regulation of actin cytoskeleton, calcium signaling pathway, oocyte meiosis, thyroid hormone synthesis, valine, leucine, isoleucine degradation, tyrosine metabolism, histidine metabolism, glyoxylate and dicarboxylate metabolism, nicotinate and nicotinamide metabolism, and glutathione metabolism, etc. Conclusion The telomere length of GV and GC in DOR patients become shorter. Totally 997 up-regulated genes and 525 downregulated genes were significantly enriched in cell division, apoptosis, and negative regulation of cell proliferation, which might cause changes in the activity of signaling pathways related to ovarian development, thereby promoting the occurrence of DOR. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
观察卵巢储备功能减退 (DOR) 患者卵泡颗粒细胞/未成熟卵母细胞端粒长度, 筛选颗粒细胞差异 表达基因, 并分析差异表达基因的生物学功能。方法 采用qPCR法检测4例DOR患者 (减退组) 和4例卵巢功能正 常者 (正常组) 颗粒细胞, 未成熟卵母细胞的端粒长度; 高通量测序分析4例减退组和4例正常组颗粒细胞差异表达 基因, 并进行生物学功能分析。结果 减退组, 正常组未成熟卵母细胞端粒长度分别为0. 935, 1. 39, 颗粒细胞端粒 长度分别为0. 915, 1. 34, 两者比较, P均<0. 05。相比于正常组, 减退组检测到997个上调基因, 525个下调基因; GO 功能分析发现, 差异表达基因显著富集涉及细胞黏附, 细胞分裂, 负向调控BMP信号通路, 凋亡过程的正调控, 细胞 增殖的负调控, ATP结合, 细胞连接, 线粒体基质, 催化活性, 蛋白酶结合, 线粒体, 线粒体内膜, 氧化还原酶活性, 对 cAMP反应等, 差异基因KEGG信号通路包括cAMP信号通路, cGMP-PKG信号通路, 胰岛素分泌, 肌动蛋白细胞骨架 的调节, 钙信号通路, 卵母细胞减数分裂, 甲状腺激素合成, 缬氨酸, 亮氨酸, 异亮氨酸降解, 酪氨酸代谢, 组氨酸代 谢, 乙醛酸酯和二羧酸酯代谢, 烟酸酯和烟酰胺代谢, 谷胱甘肽代谢等。结论 DOR患者未成熟卵母细胞和颗粒细 胞端粒长度变短, 颗粒细胞差异表达基因有997个上调基因, 525个下调基因, 主要参与细胞分裂, 细胞凋亡, 细胞增 殖负调控等信号通路, 致使卵巢发育过程相关的信号通路的活性改变, 从而促进DOR的发生. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
