دورية أكاديمية
Trehalose attenuates abdominal aortic aneurysm formation by inducing autophagy in smooth muscle cells.
| العنوان: | Trehalose attenuates abdominal aortic aneurysm formation by inducing autophagy in smooth muscle cells. |
|---|---|
| المؤلفون: | Bo JIANG, Xuan LI, Mo WANG, Guang-Xin LI, Peng-Wei REN, Yu-Qi WANG, Shi- Jie XIN, Ling-Feng QIN |
| المصدر: | Journal of Geriatric Cardiology; 2023, Vol. 20 Issue 3, p214-222, 9p |
| مصطلحات موضوعية: | ABDOMINAL aortic aneurysms, SMOOTH muscle, STAINS & staining (Microscopy), AUTOPHAGY, ANIMAL experimentation, WESTERN immunoblotting, COMPARATIVE studies, FLUORESCENT antibody technique, ELECTROCARDIOGRAPHY, TUMOR markers, DISACCHARIDES, MICE |
| مصطلحات جغرافية: | UNITED States |
| مستخلص: | BACKGROUND Trehalose is a naturally occurring disaccharide, which has been identified as an autophagy inducer and exhibits protective effect in cardiovascular diseases such as myocardial infraction and atherosclerosis. However, the functional role of trehalose in abdominal aortic aneurysm (AAA) remains undefined. METHODS To study the effect of trehalose in AAA, trehalose (1 g/kg per day) were given for 14 continuous days in a mouse model of elastase-induced abdominal aortic aneurysm. On day 14, ultrasound was performed to measure aortic diameter before the abdominal aortas were harvested and processed for further analysis. Verhoeff-Van Gieson staining and TUNEL staining were performed on paraffin sections to evaluate vascular histology and apoptosis, immunofluorescence staining and Western-blot were performed to evaluate expression of autophagy markers. RESULTS Echocardiography and in situ pictures demonstrated that trehalose attenuated infrarenal aorta dilation. Verhoeff- Van Gieson staining showed elastin degradation was improved in trehalose-treated group. Compared with vehicle-treated mice, trehalose treatment restored smooth muscle cell contractile phenotype with increased α-SMA, Calponin and Myh11 expression. Furthermore, trehalose also attenuated cell apoptosis and leukocytes infiltration. Importantly, trehalose induced autophagy with decrease SQSTM1/p62 accumulation, increased lamp2 expression and LC3B conversion. CONCLUSION Trehalose attenuated AAA progression with decreased inflammation and restored SMC contractile phenotype by inducing autophagy. These results demonstrated the therapeutic potential of trehalose in AAA. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Geriatric Cardiology is the property of Journal of Geriatric Cardiology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| قاعدة البيانات: | Complementary Index |
كن أول من يترك تعليقا!