التفاصيل البيبلوغرافية
| العنوان: |
The Intricacies of Inflammatory Bowel Disease: A Preliminary Study of Redox Biology in Intestinal Organoids. |
| المؤلفون: |
Csukovich, Georg, Huainig, Janina, Troester, Selina, Pratscher, Barbara, Burgener, Iwan Anton |
| المصدر: |
Organoids; Sep2023, Vol. 2 Issue 3, p156-164, 9p |
| مصطلحات موضوعية: |
INFLAMMATORY bowel diseases, ORGANOIDS, MITOCHONDRIA, OXIDATION-reduction reaction, HYDROGEN peroxide |
| مستخلص: |
We evaluated the redox status, precisely glutathione levels, which have a major impact in cellular detoxification and antioxidant defence in IBD-derived and healthy intestinal organoids. Therefore, we wanted to explore the differences in terms of their redox balance and mitochondrial fitness. To this end, we introduced a Grx1-roGFP2 construct into the organoids by lentiviral transduction before performing a stress assay by treating the organoids with hydrogen peroxide and examined the GSH/GSSG ratio using confocal imaging. Using ratio imaging, we could detect statistically significant differences between healthy and IBD-derived samples. To gain more insight, we also performed a GSH/GSSG assay, which directly measured glutathione levels. This analysis revealed that both organoid lines had higher levels of oxidized glutathione due to the stress treatment demonstrated by a lower GSH/GSSG ratio compared to the untreated control. Nevertheless, the results showed no significant difference between healthy and IBD-derived organoids. We further challenged organoids with hydrogen peroxide after incubation with MitoTracker® to see if mitochondrial fitness might be different in IBD-derived organoids. However, these results were also very comparable. In summary, our preliminary findings indicate that both organoid lines demonstrate a well-functioning system in terms of analysis but show no clear difference between healthy and IBD-derived samples. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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