التفاصيل البيبلوغرافية
| العنوان: |
The Enterotoxin Gene Profiles and Enterotoxin Production of Staphylococcus aureus Strains Isolated from Artisanal Cheeses in Belgium. |
| المؤلفون: |
Minutillo, Raphaëlle, Pirard, Barbara, Fatihi, Abdelhak, Cavaiuolo, Marina, Lefebvre, Donatien, Gérard, Amaury, Taminiau, Bernard, Nia, Yacine, Hennekinne, Jacques-Antoine, Daube, Georges, Clinquart, Antoine |
| المصدر: |
Foods; Nov2023, Vol. 12 Issue 21, p4019, 14p |
| مصطلحات موضوعية: |
ENTEROTOXINS, LIQUID chromatography-mass spectrometry, STAPHYLOCOCCUS aureus, FOOD poisoning, CHEESE, GENETIC profile, GOURMET foods, GENE expression |
| مصطلحات جغرافية: |
BELGIUM |
| مستخلص: |
A Staphyloccoccus aureus is one of the leading causes of food poisoning outbreaks (FPOs) worldwide. Staphylococcal food poisoning (SFP) is induced by the ingestion of food containing sufficient levels of staphylococcal enterotoxins (SEs). Currently, 33 SEs and SE-like toxins (SEls) have been described in the literature, but only five named "classical" enterotoxins are commonly investigated in FPOs due to lack of specific routine analytical techniques. The aims of this study were to (i) establish the genetic profile of strains in a variety of artisanal cheeses (n = 30) in Belgium, (ii) analyze the expression of the SE(l)s by these strains and (iii) compare the output derived from the different analytical tools. Forty-nine isolates of S. aureus were isolated from ten Belgian artisanal cheeses and were analyzed via microbiological, immunological, liquid chromatography mass spectrometry, molecular typing and genetic methods. The results indicated that classical SEs were not the dominant SEs in the Belgian artisanal cheeses that were analyzed in this study, and that all S. aureus isolates harbored at least one gene encoding a new SE(l). Among the new SE(l)s genes found, some of them code for enterotoxins with demonstrated emetic activity and ecg-enterotoxins. It is worth noting that the involvement of some of these new SEs has been demonstrated in SFP outbreaks. Thus, this study highlighted the importance of the development of specific techniques for the proper investigation of SFP outbreaks. [ABSTRACT FROM AUTHOR] |
|
Copyright of Foods is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder