التفاصيل البيبلوغرافية
| العنوان: |
Biochemical and genetic studies define the functions of methylthiotransferases in methanogenic and methanotrophic archaea. |
| المؤلفون: |
Boswinkle, Kaleb, Thuc-Anh Dinh, Allen, Kylie D. |
| المصدر: |
Frontiers in Microbiology; 2023, p1-14, 14p |
| مصطلحات موضوعية: |
TRANSFER RNA, ARCHAEBACTERIA, RIBOSOMAL proteins, RADICALS (Chemistry), ADENOSYLMETHIONINE, DELETION mutation |
| مستخلص: |
Methylthiotransferases (MTTases) are radical S-adenosylmethionine (SAM) enzymes that catalyze the addition of a methylthio (-SCH3) group to an unreactive carbon center. These enzymes are responsible for the production of 2-methylthioadenosine (ms²A) derivatives found at position A37 of select tRNAs in all domains of life. Additionally, some bacteria contain the RimO MTTase that catalyzes the methylthiolation of the S12 ribosomal protein. Although the functions of MTTases in bacteria and eukaryotes have been established via detailed genetic and biochemical studies, MTTases from the archaeal domain of life are understudied and the substrate specificity determinants of MTTases remain unclear. Here, we report the in vitro enzymatic activities of an MTTase (C4B56_06395) from a thermophilic Ca. Methanophagales anaerobic methanotroph (ANME) as well as the MTTase from a hyperthermophilic methanogen – MJ0867 from Methanocaldococcus jannaschii. Both enzymes catalyze the methylthiolation of N6 -threonylcarbamoyladenosine (t6A) and N6-hydroxynorvalylcarbamoyladenosine (hn6A) residues to produce 2-methylthio-N6-threonylcarbamoyladenosine (ms² t6 A) and 2-methylthio-N6-hydroxynorvalylcarbamoyladenosine (ms² hn6A), respectively. To further assess the function of archaeal MTTases, we analyzed select tRNA modifications in a model methanogen – Methanosarcina acetivorans – and generated a deletion of the MTTase-encoding gene (MA1153). We found that M. acetivorans produces ms2 hn6 A in exponential phase of growth, but does not produce ms² t6 A in detectable amounts. Upon deletion of MA1153, the ms²A modification was absent, thus confirming the function of MtaB-family MTTases in generating ms² hn6 A modified nucleosides in select tRNAs. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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