دورية أكاديمية
2.5D mass spectrometry imaging of N-glycans in esophageal adenocarcinoma and precursor lesions.
| العنوان: | 2.5D mass spectrometry imaging of N-glycans in esophageal adenocarcinoma and precursor lesions. |
|---|---|
| المؤلفون: | Vos, D. R. N., Meijer, S. L., Pouw, R. E., Ellis, S. R., Heeren, R. M. A., Balluff, B. |
| المصدر: | Frontiers in Analytical Science; 11/29/2022, p1-12, 12p |
| مصطلحات موضوعية: | MASS spectrometry, BARRETT'S esophagus, SURGICAL excision, ENDOSCOPIC surgery, ADENOCARCINOMA |
| مستخلص: | Glycosylation plays an important role in the progression of esophageal adenocarcinoma (EAC). Being able to image these glycosylation changes directly in endoscopic resection specimens could provide useful insights into the molecular mechanisms of the disease progression and potential markers for EACstaging. For this purpose, both3Dand 2.5D matrix-assisted laser/desorption ionization (MALDI) mass spectrometry imaging (MSI) have been employed in this study to investigate glycosidase-cleaved N-glycans in a total of24 formalin-fixed paraffin-embedded esophageal local excision specimens spanning all stages of disease progression, namely from non-dysplastic Barrett's esophagus to metastatic EAC. 3D-MSI was first used to estimate the number of sections needed to sufficiently cover the molecular heterogeneity of each stage of progression. This analysis showed that a total of four sections out of 20 were sufficient. This subset of four sections was measured for all remaining specimens and is called 2.5D-MSI. Subsequent analyses of the 2.5D-MSI datasets revealed significant elevations offivehigh-mannoseN-glycans (Man3,Man4,Man6,Man7, and Man8) in EAC and three complex (Hex6HexNAc5, Hex6HexNAc5NeuAc1, Hex7HexNAc6) N-glycans in metastatic EAC as compared to previous stages of the disease. The augmented levels of these glycans in EAC could be explained by publically available gene expression data of enzymes involved in glycan synthesis and processing. As the role of glycosylation is gaining more interest in MSI and cancer research, our results show the added value of combining localized N-glycan levels, as provided by MSI, with gene expression to gain a deeper understanding of the mechanisms behind N-glycan changes. This gives evidence at multiple levels that specific N-glycosylation plays an important role during progression of dysplasia to EAC and could play a role in patient surveillance. [ABSTRACT FROM AUTHOR] |
| Copyright of Frontiers in Analytical Science is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| قاعدة البيانات: | Complementary Index |
Full Text Finder كن أول من يترك تعليقا!